Journal Article

Curcumin pretreatment and post-treatment both improve the antioxidative ability of neurons with oxygen-glucose deprivation

Jing-xian Wu Lu-yu Zhang Yan-lin Chen Shan-shan Yu Yong Zhao Jing Zhao

2015

Overview

Recent studies have shown that induced expression of endogenous antioxidative enzymes thr- ough activation of the antioxidant response element/nuclear factor erythroid 2-related factor 2 （Nrf2） pathway may be a neuroprotective strategy. In this study, rat cerebral cortical neurons cultured in vitro were pretreated with 10 ktM curcumin or post-treated with 5 pM curcumin, respectively before or after being subjected to oxygen-glucose deprivation and reoxygenation for 24 hours. Both pretreatment and post-treatment resulted in a significant decrease of cell injury as indicated by propidium iodide/Hoechst 33258 staining, a prominent increase of Nrf2 protein expression as indicated by western blot analysis, and a remarkable increase of protein expression and enzyme activity in whole cell lysates of thioredoxin before ischemia, after ischemia, and after reoxygenation. In addition, post-treatment with curcumin inhibited early DNA/RNA oxidation as indicated by immunocytochemistry and increased nuclear Nrf2 protein by inducing nuclear accumulation of Nrf2. These findings suggest that curcumin activates the expression of thi- oredoxin, an antioxidant protein in the Nrf2 pathway, and protects neurons from death caused by oxygen-glucose deprivation in an in vitro model of ischemia/reperfusion. We speculate that pharmacologic stimulation of antioxidant gene expression may be a promising approach to neu- roprotection after cerebral ischemia.

• Publisher: Medknow Publications and Media Pvt. Ltd,Medknow Publications & Media Pvt. Ltd,Department of Pathology, Chongqing Medical University, Chongqing, China%Molecular Medicine and Cancer Research Center, Chongqing Medical University, Chongqing, China%Institute of Neuroscience, Chongqing Medical University, Chongqing, China,Medknow Publications & Media Pvt Ltd,Wolters Kluwer Medknow Publications
• Subject: acupuncture / Alzheimer′s disease / Antioxidants / Antioxidants (Nutrients) / apoptosis / apparent diffusion coefficient / astrocytes / auranofin / average combined score / blood-nerve barrier / bone marrow mesenchymal stem cells / brain injury / caspase-3 / cell transplantation / cerebral hemorrhage / cerebral ischemia / Chinese herbal formula / CHOP / cisplatin / cochlea / cognitive neuroscience / consciousness / cortical neurons / Curcumin / DAPT / dentate gyrus / dextromethorphan / differentiation / diffusion tensor imaging / disorders of consciousness / electroacupuncture therapy / electrophysiology / endoplasmic reticulum stress / evaluation / Evans blue tracer / event-related potential / Experiments / fiber tractography / fractional anisotropy / gene therapy / glaucoma / Glucose / GRP78 / Health aspects / Hes1 / hippocampus / hypothermia / immunohistochemistry / inflammation / intraocular pressure / Ischemia / ischemia/reperfusion injury / JNK3 / Laboratory animals / laser photocoagulation / lipid peroxidation / lumbar puncture / magnetic guidance / magnetic resonance image / magnetic resonance imaging / malondialdehyde / microglia / minimally conscious state / motor learning / nanocarrier / nerve growth factor receptor / nerve regeneration / neural degeneration / neural regeneration / neural stem cells / neurodegeneration / neuroinflammation / Neurons / neuroprotection / notch signaling pathway / Notch1 / NSFC grant / NSFC grants / nutritional state / ocular hypertension / ongluo Jiunao injection / ototoxicity / Oxidative stress / oxygen-glucose deprivation / P300 / paraquat / Penicillin / peripheral nerve injury / poisoning / polyethyleneimine-polyethylene glycol / post-treatment / PP2 / pretreatment / primary cell culture / primary motor area / prognosis / proliferation / Proteins / rats / reactive oxygen species / real-time quantitative PCR / regeneration / retinal ganglion cell / rosiglitazone / sciatic nerve / SMAD3 / spinal cord / spinal cord injury / spinal cord transection / spiral ganglion cells / Src kinase / superparamagnetic iron oxide / survival curve / tamoxifen / task difficulty / Tneurotrophic factor / transcranial direct current stimulation / transforming growth factor β1 / trauma / type 1 diabetes / ulinastatin / unconsciousness / visuomotor coordination / western blot assay / Western印迹 / X-linked inhibitor of apoptosis protein / Xuesai Tong / 后处理 / 姜黄素 / 抗氧化能力 / 皮质神经元 / 缺氧 / 缺血/再灌注 / 预处理