Journal Article

Electrospraying Oxygen-Generating Microparticles for Tissue Engineering Applications

Domingues Stocco, Thiago, Vieira, Ewerton, Furtini, Josy, Silva-Filho, Edson, Lobo, Anderson, Ruiz-Esparza, Guillermo, Marciano, Fernanda, Ícaro Sousa Morais, Alan, Silva, Heurison, Corat, Marcus, De-Paula, Mirian, Afewerki, Samson, Wang, Xichi, Viana, Bartolomeu

2020

Overview

The facile preparation of oxygen-generating microparticles (M) consisting of Polycaprolactone (PCL), Pluronic F-127, and calcium peroxide (CPO) (PCL-F-CPO-M) fabricated through an electrospraying process is disclosed. The biological study confirmed the positive impact from the oxygen-generating microparticles on the cell growth with high viability. The presented technology could work as a prominent tool for various tissue engineering and biomedical applications. The oxygen-generated microparticles fabricated through electrospraying processes were thoroughly characterization through various methods such as X-ray diffraction (XRD), Fourier Transform Infrared Spectroscopy (FTIR) analysis, and scanning electron microscopy (SEM)/SEM-Energy Dispersive Spectroscopy (EDS) analysis. The analyses confirmed the presence of the various components and the porous structure of the microparticles. Spherical shape with spongy characteristic microparticles were obtained with negative charge surface (ζ = -16.9) and a size of 17.00 ± 0.34 μm. Furthermore, the biological study performed on rat chondrocytes demonstrated good cell viability and the positive impact of increasing the amount of CPO in the PCL-F-CPO-M. This technological platform could work as an important tool for tissue engineering due to the ability of the microparticles to release oxygen in a sustained manner for up to 7 days with high cell viability.

• Publisher: Dove Medical Press Limited,Dove Press,Dove,Dove Medical Press
• Subject: Animals / Biocompatible Materials - chemistry / Biomedical engineering / calcium peroxide / cartilage / Cell Culture Techniques / Cell Proliferation / Cell Survival - drug effects / Characterization / Chondrocytes - drug effects / Electrochemical Techniques / Electron microscopy / electrospraying / Infrared spectroscopy / Microscopy / Original Research / Oxygen - chemistry / Oxygen - pharmacokinetics / oxygen-generating-microparticles / Peroxides / Peroxides - chemistry / Poloxamer - chemistry / Polycaprolactone / Polyesters - chemistry / Porosity / Rats, Wistar / Spectrometry, X-Ray Emission / Spectroscopy / Spectroscopy, Fourier Transform Infrared / Technology / Tissue engineering / Tissue Engineering - methods / X-Ray Diffraction