Journal Article

Activation of Symbiosis Signaling by Arbuscular Mycorrhizal Fungi in Legumes and Rice

Miller, J. Benjamin, Ané, Jean-Michel, Maillet, Fabienne, Denarié, Jean, European Research Council as “SYMBIOSIS,” the Bill and Melinda Gates Foundation, the Biotechnology and Biological Sciences Research Council as BB/J004553/1, Labex Arcane, ICMG as FR2607, and the Gatsby Charitable Foundation as a Sainsbury PhD fellowship, Laboratoire des interactions plantes micro-organismes (LIPM) ; Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS), University of Wisconsin-Madison, Sun, Jongho, Fort, Sébastien, Venkateshwaran, Muthusubramanian, Wiley-Kalil, Audrey, Cottaz, Sylvain, Centre de Recherches sur les Macromolécules Végétales (CERMAV) ; Université Joseph Fourier - Grenoble 1 (UJF)-Institut de Chimie - CNRS Chimie (INC-CNRS)-Centre National de la Recherche Scientifique (CNRS), Gobbato, Enrico, E, Samain, Eric, Granqvist, Emma, John Innes Centre [Norwich] ; Biotechnology and Biological Sciences Research Council (BBSRC), Oldroyd, Giles E.D, Morris, Richard, W

2015

Overview

Establishment of arbuscular mycorrhizal interactions involves plant recognition of diffusible signals from the fungus, including lipochitooligosaccharides (LCOs) and chitooligosaccharides (COs). Nitrogen-fixing rhizobial bacteria that associate with leguminous plants also signal to their hosts via LCOs, the so-called Nod factors. Here, we have assessed the induction of symbiotic signaling by the arbuscular mycorrhizal (Myc) fungal-produced LCOs and COs in legumes and rice (Oryza sativa). We show that Myc-LCOs and tetra-acetyl chitotetraose (CO4) activate the common symbiosis signaling pathway, with resultant calcium oscillations in root epidermal cells of Medicago truncatula and Lotus japonicus. The nature of the calcium oscillations is similar for LCOs produced by rhizobial bacteria and by mycorrhizal fungi; however, Myc-LCOs activate distinct gene expression. Calcium oscillations were activated in rice atrichoblasts by CO4, but not the Myc-LCOs, whereas a mix of CO4 and Myc-LCOs activated calcium oscillations in rice trichoblasts. In contrast, stimulation of lateral root emergence occurred following treatment with Myc-LCOs, but not CO4, in M. truncatula, whereas both Myc-LCOs and CO4 were active in rice. Our work indicates that legumes and non-legumes differ in their perception of Myc-LCO and CO signals, suggesting that different plant species respond to different components in the mix of signals produced by arbuscular mycorrhizal fungi.

• Publisher: American Society of Plant Biologists (ASPB),CCSD,American Society of Plant Biologists
• Subject: Botanics / Calcium / Calcium Signaling - drug effects / Chitin - analogs & derivatives / Chitin - pharmacology / Chitosan / Ecology, environment / Fungi / Gene Expression Regulation, Plant - drug effects / Glucuronidase - metabolism / Legumes / Life Sciences / Lipopolysaccharides - pharmacology / Lotus - microbiology / Lotus japonicus / Medicago truncatula / Medicago truncatula - drug effects / Medicago truncatula - genetics / Medicago truncatula - microbiology / Microbiology and Parasitology / Molecular Sequence Data / Molecules / Mycology / Mycorrhizae - drug effects / Mycorrhizae - physiology / mycorrhizal fungi / Oligosaccharides - pharmacology / Oryza - drug effects / Oryza - genetics / Oryza - microbiology / Oryza sativa / Plant cells / Plant roots / Plants / Rice / Seedlings / Seedlings - drug effects / Seedlings - microbiology / Signal Transduction - drug effects / Symbiosis / Symbiosis - drug effects / Vegetal Biology
• ISBN: 0003546402000