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Journal Article
Translational termination without a stop codon
2016
Overview
Ribosomes stall when they encounter the end of messenger RNA (mRNA) without an in-frame stop codon. In bacteria, these \"nonstop\" complexes can be rescued by alternative ribosome-rescue factor A (ArfA). We used electron cryomicroscopy to determine structures of ArfA bound to the ribosome with 3'-truncated mRNA, at resolutions ranging from 3.0 to 3.4 angstroms. ArfA binds within the ribosomal mRNA channel and substitutes for the absent stop codon in the A site by specifically recruiting release factor 2 (RF2), initially in a compact preaccommodated state. A similar conformation of RF2 may occur on stop codons, suggesting a general mechanism for release-factor-mediated translational termination in which a conformational switch leads to peptide release only when the appropriate signal is present in the A site.
Publisher
American Association for the Advancement of Science,The American Association for the Advancement of Science
Subject
/ Bacteria
/ Channels
/ Escherichia coli - metabolism
/ Escherichia coli Proteins - chemistry
/ Escherichia coli Proteins - metabolism
/ Escherichia coli Proteins - ultrastructure
/ Peptide Chain Termination, Translational
/ Peptide Termination Factors - chemistry
/ Peptide Termination Factors - metabolism
/ Peptides
/ Plugs
/ RNA
/ RNA-Binding Proteins - chemistry
/ RNA-Binding Proteins - metabolism
/ RNA-Binding Proteins - ultrastructure
/ Stall
/ Switches
