Asset Details
Do you wish to reserve the book?
In vitro evaluation of simulated stereotactic radiotherapy for wet age-related macular degeneration on three different cell lines
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
In vitro evaluation of simulated stereotactic radiotherapy for wet age-related macular degeneration on three different cell lines
Do you wish to request the book?
In vitro evaluation of simulated stereotactic radiotherapy for wet age-related macular degeneration on three different cell lines
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
In vitro evaluation of simulated stereotactic radiotherapy for wet age-related macular degeneration on three different cell lines
2021
Overview
Low energy stereotactic radiotherapy has been proposed for the treatment of neovascular age related macular degeneration. We investigated the in vitro effect of the radiotherapy on pericytes, retinal pigment epithelium and endothelial cells. Primary human retinal pigment epithelium cells, human umbilical vein endothelial cells and human pericytes from Placenta were cultivated. In a pairwise protocol, one plate was irradiated at a dose of 16 Gy, while the second plate served as a non-irradiated control. Thereafter, cells were cultivated either in serum-free (non-permissive) or serum-stimulated (permissive) conditions. A life/dead assay, an XTT and a BrdU assay were performed up to 7 days after irradiation. No cell death occurred at any timepoint in any cell line after treatment nor in the control. Compared to the unirradiated controls, cell viability and metabolic activity were significantly reduced in irradiated cells in the XTT assay, except for non-permissive RPE cells. In the BrdU assay, proliferation was inhibited. While no cell death was detected in vitro, viability and proliferative capacity of all cell lines were significantly reduced. Therefore, it seems that low energy stereotactic radiotherapy inhibits angiogenesis without a direct induction of apoptosis but influencing microvascular function and stability.
Publisher
Springer Science and Business Media LLC,Nature Publishing Group UK,Nature Publishing Group,Nature Portfolio
Subject
/ 692/699
/ Age
/ Apoptosis - radiation effects
/ Cell Proliferation - radiation effects
/ Cell Survival - radiation effects
/ Diseases
/ Human Umbilical Vein Endothelial Cells - radiation effects
/ Humanities and Social Sciences
/ Humans
/ info:eu-repo/classification/ddc/610
/ Macular Degeneration - pathology
/ Medicine
/ Pericytes - radiation effects
/ Placenta
/ Q
/ R
/ Retina
/ Retinal Pigment Epithelium - cytology
/ Retinal Pigment Epithelium - radiation effects
/ Science
