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Determination of d- and l-Amino Acids in Garlic Foodstuffs by Liquid Chromatography–Tandem Mass Spectrometry
by
Onozato, Mayu
, Fukushima, Takeshi
, Nakanoue, Haruna
, Umino, Maho
, Sakamoto, Tatsuya
in
Amino Acid Sequence
/ Amino acids
/ Amino Acids - analysis
/ black garlic
/ chiral derivatization
/ Chromatography
/ Chromatography, Liquid
/ CIMa-OSu
/ Communication
/ d -aspartic acid
/ d -serine
/ Diabetes
/ Fermentation
/ Garlic
/ Ions
/ Mass spectrometry
/ Methanol
/ NMR
/ Nuclear magnetic resonance
/ Reagents
/ Scientific imaging
/ Tandem Mass Spectrometry
2023
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Determination of d- and l-Amino Acids in Garlic Foodstuffs by Liquid Chromatography–Tandem Mass Spectrometry
by
Onozato, Mayu
, Fukushima, Takeshi
, Nakanoue, Haruna
, Umino, Maho
, Sakamoto, Tatsuya
in
Amino Acid Sequence
/ Amino acids
/ Amino Acids - analysis
/ black garlic
/ chiral derivatization
/ Chromatography
/ Chromatography, Liquid
/ CIMa-OSu
/ Communication
/ d -aspartic acid
/ d -serine
/ Diabetes
/ Fermentation
/ Garlic
/ Ions
/ Mass spectrometry
/ Methanol
/ NMR
/ Nuclear magnetic resonance
/ Reagents
/ Scientific imaging
/ Tandem Mass Spectrometry
2023
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Determination of d- and l-Amino Acids in Garlic Foodstuffs by Liquid Chromatography–Tandem Mass Spectrometry
by
Onozato, Mayu
, Fukushima, Takeshi
, Nakanoue, Haruna
, Umino, Maho
, Sakamoto, Tatsuya
in
Amino Acid Sequence
/ Amino acids
/ Amino Acids - analysis
/ black garlic
/ chiral derivatization
/ Chromatography
/ Chromatography, Liquid
/ CIMa-OSu
/ Communication
/ d -aspartic acid
/ d -serine
/ Diabetes
/ Fermentation
/ Garlic
/ Ions
/ Mass spectrometry
/ Methanol
/ NMR
/ Nuclear magnetic resonance
/ Reagents
/ Scientific imaging
/ Tandem Mass Spectrometry
2023
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Determination of d- and l-Amino Acids in Garlic Foodstuffs by Liquid Chromatography–Tandem Mass Spectrometry
Journal Article
Determination of d- and l-Amino Acids in Garlic Foodstuffs by Liquid Chromatography–Tandem Mass Spectrometry
2023
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Overview
Black garlic is currently attracting interest as a health food and constituent of commercial supplements; however, no data regarding the d-amino acids within black garlic have been reported. Therefore, the amino acid compositions of methanol extracts from fresh and black garlic were compared herein. We investigated the contents of the d- and l-forms of amino acids in commercial fresh, black, and freeze-dried garlic foodstuffs by liquid chromatography–tandem mass spectrometry (LC–MS/MS) using a pre-column chiral derivatization reagent, succinimidyl 2-(3-((benzyloxy)carbonyl)-1-methyl-5-oxoimidazolidin-4-yl) acetate. Several d-amino acids, namely, the d-forms of Asn, Ala, Ser, Thr, Glu, Asp, Pro, Arg, Phe, Orn, Lys, and Tyr, were observed in the methanol extract of black garlic, whereas only d-Ala was detected in that of fresh garlic foodstuffs. These data suggest that several d-amino acids can be produced during fermentation for preparing black garlic.
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