Journal Article

Adipose-Derived Mesenchymal Stem Cell Protects Kidneys against Ischemia-Reperfusion Injury through Suppressing Oxidative Stress and Inflammatory Reaction

Chung, Sheng-Ying, Kao, Ying-Hsien, Sun, Cheuk-Kwan, Lin, Yu-Chun, Chang, Li-Teh, Leu, Steve, Chua, Sarah, Yen, Chia-Hung, Chen, Yung-Lung, Yip, Hon-Kan, Shao, Pei-Lin, Tsai, Tzu-Hsien, Chen, Yen-Ta, Chang, Kuan-Cheng

2011

Overview

Background Reactive oxygen species are important mediators exerting toxic effects on various organs during ischemia-reperfusion (IR) injury. We hypothesized that adipose-derived mesenchymal stem cells (ADMSCs) protect the kidney against oxidative stress and inflammatory stimuli in rat during renal IR injury. Methods Adult male Sprague-Dawley (SD) rats (n = 24) were equally randomized into group 1 (sham control), group 2 (IR plus culture medium only), and group 3 (IR plus immediate intra-renal administration of 1.0 × 10 6 autologous ADMSCs, followed by intravenous ADMSCs at 6 h and 24 h after IR). The duration of ischemia was 1 h, followed by 72 hours of reperfusion before the animals were sacrificed. Results Serum creatinine and blood urea nitrogen levels and the degree of histological abnormalities were markedly lower in group 3 than in group 2 (all p < 0.03). The mRNA expressions of inflammatory, oxidative stress, and apoptotic biomarkers were lower, whereas the anti-inflammatory, anti-oxidative, and anti-apoptotic biomarkers were higher in group 3 than in group 2 (all p < 0.03). Immunofluorescent staining showed a higher number of CD31+, von Willebrand Factor+, and heme oxygenase (HO)-1+ cells in group 3 than in group 2 (all p < 0.05). Western blot showed notably higher NAD(P)H quinone oxidoreductase 1 and HO-1 activities, two indicators of anti-oxidative capacity, in group 3 than those in group 2 (all p < 0.04). Immunohistochemical staining showed higher glutathione peroxidase and glutathione reductase activities in group 3 than in group 2 (all p < 0.02) Conclusion ADMSC therapy minimized kidney damage after IR injury through suppressing oxidative stress and inflammatory response.

• Publisher: BioMed Central,BioMed Central Ltd,BMC
• Subject: Adipose Tissue - cytology / Animals / Antioxidants - metabolism / Apoptosis - genetics / Biomedical and Life Sciences / Biomedicine / Blood Urea Nitrogen / Care and treatment / Cell / Creatinine - blood / Creatinine - urine / Flow Cytometry / Fluorescent Antibody Technique / Gene Expression Regulation / Health aspects / Inflammation - complications / Inflammation - genetics / Inflammation - prevention & control / Ischemia / Kidney - metabolism / Kidney - pathology / Kidney diseases / Kidney Tubules - metabolism / Kidney Tubules - pathology / Male / Medicine/Public Health / Mesenchymal Stem Cell Transplantation / Mesenchymal Stromal Cells - cytology / Oxidative stress / Oxidative Stress - genetics / Platelet Endothelial Cell Adhesion Molecule-1 - metabolism / Rats / Rats, Sprague-Dawley / Reperfusion Injury - blood / Reperfusion Injury - pathology / Reperfusion Injury - prevention & control / Reperfusion Injury - urine / RNA, Messenger - genetics / RNA, Messenger - metabolism / Stem cells / tissue and gene therapy / von Willebrand Factor - metabolism