Asset Details
Do you wish to reserve the book?
Serology of Paracoccidioidomycosis Due to Paracoccidioides lutzii
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Serology of Paracoccidioidomycosis Due to Paracoccidioides lutzii
Do you wish to request the book?
Serology of Paracoccidioidomycosis Due to Paracoccidioides lutzii
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Serology of Paracoccidioidomycosis Due to Paracoccidioides lutzii
2014
Overview
Paracoccidioides lutzii is a new agent of paracoccidioidomycosis (PCM) and has its epicenter localized to the Central-West region of Brazil. Serological diagnosis of PCM caused by P. lutzii has not been established. This study aimed to develop new antigenic preparations from P. lutzii and to apply them in serological techniques to improve the diagnosis of PCM due to P. lutzii. Paracoccidioides lutzii exoantigens, cell free antigen (CFA), and a TCA-precipitated antigen were evaluated in immunodiffusion (ID) tests using a total of 89 patient sera from the Central-West region of Brazil. Seventy-two sera were defined as reactive for P. brasiliensis using traditional antigens (AgPbB339 and gp43). Non-reactive sera for traditional antigens (n = 17) were tested with different P. lutzii preparations and P. lutzii CFA showed 100% reactivity. ELISA was found to be a very useful test to titer anti-P. lutzii antibodies using P. lutzii-CFA preparations. Sera from patients with PCM due to P. lutzii presented with higher antibody titers than PCM due to P. brasiliensis and heterologous sera. In western blot, sera from patients with PCM due to P. lutzii were able to recognize antigenic molecules from the P. lutzii-CFA antigen, but sera from patients with PCM due to P. brasiliensis could not recognize any P. lutzii molecules. Due to the facility of preparing P. lutzii CFA antigens we recommend its use in immunodiffusion tests for the diagnosis of PCM due to P. lutzii. ELISA and western blot can be used as complementary tests.
Publisher
Public Library of Science,Public Library of Science (PLoS)
Subject
/ Aged
/ Antigens
/ Antigens, Fungal - immunology
/ Antigens, Fungal - isolation & purification
/ Brazil
/ Enzyme-Linked Immunosorbent Assay
/ Female
/ Humans
/ Male
/ Paracoccidioides - immunology
/ Paracoccidioides - isolation & purification
/ Paracoccidioides brasiliensis
/ Paracoccidioidomycosis - diagnosis
/ Paracoccidioidomycosis - immunology
/ Paracoccidioidomycosis - microbiology
/ Patients
/ Research and Analysis Methods
/ Serology
