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Structure of the transition state for the binding of c-Myb and KIX highlights an unexpected order for a disordered system
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Structure of the transition state for the binding of c-Myb and KIX highlights an unexpected order for a disordered system
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Journal Article
Structure of the transition state for the binding of c-Myb and KIX highlights an unexpected order for a disordered system
2013
Overview
A classical dogma of molecular biology dictates that the 3D structure of a protein is necessary for its function. However, a considerable fraction of the human proteome, although functional, does not adopt a defined folded state under physiological conditions. These intrinsically disordered proteins tend to fold upon binding to their partners with a molecular mechanism that is elusive to experimental characterization. Indeed, although many hypotheses have been put forward, the functional role (if any) of disorder in these intrinsically denatured systems is still shrouded in mystery. Here, we characterize the structure of the transition state of the binding-induced folding in the reaction between the KIX domain of the CREB-binding protein and the transactivation domain of c-Myb. The analysis, based on the characterization of a series of conservative site-directed mutants, reveals a very high content of native-like structure in the transition state and indicates that the recognition between KIX and c-Myb is geometrically precise. The implications of our results in the light of previous work on intrinsically unstructured systems are discussed.
Publisher
National Academy of Sciences,National Acad Sciences
Subject
/ Biochemistry, Molecular Biology
/ CREB-Binding Protein - chemistry
/ CREB-Binding Protein - metabolism
/ DNA
/ Humans
/ Intrinsically Disordered Proteins
/ Intrinsically Disordered Proteins - chemistry
/ Intrinsically Disordered Proteins - genetics
/ Intrinsically Disordered Proteins - metabolism
/ Kinetics
/ Ligands
/ mutants
/ Mutation
/ Protein Interaction Domains and Motifs
/ Proteins
/ proteome
/ Proto-Oncogene Proteins c-myb
/ Proto-Oncogene Proteins c-myb - chemistry
/ Proto-Oncogene Proteins c-myb - genetics
/ Proto-Oncogene Proteins c-myb - metabolism
/ Recombinant Proteins - chemistry
/ Recombinant Proteins - genetics
