Journal Article

Death effector domain-containing herpesvirus and poxvirus proteins inhibit both Fas- and TNFR1-induced apoptosis

Bertin, J. (National Institutes of Health, Bethesda, MD.), Wang, Y, Ottilie, S, Wang, G.H, Martin, D.A, Alnemri, E.S, Armstrong, R.C, Senkevich, T.G, Banks, S, Moss, B

1997

Overview

To identify novel antiapoptotic proteins encoded by DNA viruses, we searched viral genomes for proteins that might interfere with Fas and TNFR1 apoptotic signaling pathways. We report here that equine herpesvirus type 2 E8 protein and molluscum contagiosum virus MC159 protein both show sequence similarity to the death effector domains (DEDs) of the Fas/TNFR1 signaling components FADD and caspase-8. Yeast two-hybrid analysis revealed that E8 protein interacted with the caspase-8 prodomain whereas MC159 protein interacted with FADD. Furthermore, expression of either E8 protein or MC159 protein protected cells from Fas- and TNFR1-induced apoptosis indicating that certain herpes-viruses and poxviruses use DED-mediated interactions to interfere with apoptotic signaling pathways. These findings identify a novel control point exploited by viruses to regulate Fas- and TNFR1-mediated apoptosis

• Publisher: National Academy of Sciences of the United States of America,National Acad Sciences,National Academy of Sciences,The National Academy of Sciences of the USA
• Subject: Adaptor Proteins, Signal Transducing / Amino Acid Sequence / amino acid sequences / Antigens, CD / Antigens, CD - metabolism / Apoptosis / Apoptosis - physiology / Base Sequence / binding proteins / Biochemistry / Biological Sciences / Carrier Proteins / Carrier Proteins - metabolism / Cell lines / CITOQUINAS / COMPOSICION QUIMICA / COMPOSITION CHIMIQUE / CULTIVO DE CELULAS / CULTURE DE CELLULE / Cysteine Endopeptidases / Cysteine Endopeptidases - metabolism / CYTOKINE / Deoxyribonucleic acid / DNA / DNA Viruses / fas Receptor / fas Receptor - metabolism / Fas-Associated Death Domain Protein / HeLa cells / Herpesviridae / HERPESVIRUS EQUIN / HERPESVIRUS EQUINO / Infections / INHIBICION / INHIBITION / metabolism / Models, Biological / Molecular Sequence Data / Molluscum contagiosum virus / Mollusks / physiology / Plasmids / POXVIRIDAE / Protein Binding / PROTEINAS / PROTEINAS AGLUTINANTES / PROTEINE / PROTEINE DE LIAISON / Proteins / Receptors, Tumor Necrosis Factor / Receptors, Tumor Necrosis Factor - metabolism / Receptors, Tumor Necrosis Factor, Type I / Sequence Homology, Amino Acid / Signal Transduction / T lymphocytes / tumor necrosis factors / viral proteins / Viral Proteins - metabolism / VIRUS DE LOS ANIMALES / VIRUS DES ANIMAUX / Viruses / Yeasts