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Trogocytosis of peptide–MHC class II complexes from dendritic cells confers antigen-presenting ability on basophils
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Trogocytosis of peptide–MHC class II complexes from dendritic cells confers antigen-presenting ability on basophils
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Journal Article
Trogocytosis of peptide–MHC class II complexes from dendritic cells confers antigen-presenting ability on basophils
2017
Overview
Th2 immunity plays important roles in both protective and allergic responses. Nevertheless, the nature of antigen-presenting cells responsible for Th2 cell differentiation remains ill-defined compared with the nature of the cells responsible for Th1 and Th17 cell differentiation. Basophils have attracted attention as a producer of Th2-inducing cytokine IL-4, whereas their MHC class II (MHC-II) expression and function as antigen-presenting cells are matters of considerable controversy. Here we revisited the MHC-II expression on basophils and explored its functional relevance in Th2 cell differentiation. Basophils generated in vitro from bone marrow cells in culture with IL-3 plus GM-CSF displayed MHC-II on the cell surface, whereas those generated in culture with IL-3 alone did not. Of note, these MHC-II–expressing basophils showed little or no transcription of the corresponding MHC-II gene. The GM-CSF addition to culture expanded dendritic cells (DCs) other than basophils. Coculture of basophils and DCs revealed that basophils acquired peptide–MHC-II complexes from DCs via cell contact-dependent trogocytosis. The acquired complexes, together with CD86, enabled basophils to stimulate peptide-specific T cells, leading to their proliferation and IL-4 production, indicating that basophils can function as antigen-presenting cells for Th2 cell differentiation. Transfer of MHC-II from DCs to basophils was also detected in draining lymph nodes of mice with atopic dermatitis-like skin inflammation. Thus, the present study defined the mechanism by which basophils display MHC-II on the cell surface and appears to reconcile some discrepancies observed in previous studies.
Publisher
National Academy of Sciences
Subject
/ Animals
/ Antigen Presentation - immunology
/ Antigen-Presenting Cells - immunology
/ Antigens
/ Calcitriol - analogs & derivatives
/ Dendritic Cells - immunology
/ Dermatitis, Contact - immunology
/ Dermatitis, Contact - pathology
/ Genes, MHC Class II - drug effects
/ Histocompatibility Antigens Class II - biosynthesis
/ Histocompatibility Antigens Class II - genetics
/ Histocompatibility Antigens Class II - immunology
/ Immunological Synapses - immunology
/ Mice
/ Peptide Fragments - immunology
/ Peptides
