Journal Article

Selective Chemical Inhibition of agr Quorum Sensing in Staphylococcus aureus Promotes Host Defense with Minimal Impact on Resistance

Sully, Erin K., Femling, Jon K., Gresham, Hattie D., Elmore, Bradley O., Horswill, Alexander R., Gray, Brian M., Cheung, Ambrose L., DeLeo, Frank R., Sklar, Larry A., Edwards, Bruce S., Alexander, Susan M., Otto, Michael, Hall, Pamela R., Malachowa, Natalia

2014

Overview

Bacterial signaling systems are prime drug targets for combating the global health threat of antibiotic resistant bacterial infections including those caused by Staphylococcus aureus. S. aureus is the primary cause of acute bacterial skin and soft tissue infections (SSTIs) and the quorum sensing operon agr is causally associated with these. Whether efficacious chemical inhibitors of agr signaling can be developed that promote host defense against SSTIs while sparing the normal microbiota of the skin is unknown. In a high throughput screen, we identified a small molecule inhibitor (SMI), savirin (S. aureus virulence inhibitor) that disrupted agr-mediated quorum sensing in this pathogen but not in the important skin commensal Staphylococcus epidermidis. Mechanistic studies employing electrophoretic mobility shift assays and a novel AgrA activation reporter strain revealed the transcriptional regulator AgrA as the target of inhibition within the pathogen, preventing virulence gene upregulation. Consistent with its minimal impact on exponential phase growth, including skin microbiota members, savirin did not provoke stress responses or membrane dysfunction induced by conventional antibiotics as determined by transcriptional profiling and membrane potential and integrity studies. Importantly, savirin was efficacious in two murine skin infection models, abating tissue injury and selectively promoting clearance of agr+ but not Δagr bacteria when administered at the time of infection or delayed until maximal abscess development. The mechanism of enhanced host defense involved in part enhanced intracellular killing of agr+ but not Δagr in macrophages and by low pH. Notably, resistance or tolerance to savirin inhibition of agr was not observed after multiple passages either in vivo or in vitro where under the same conditions resistance to growth inhibition was induced after passage with conventional antibiotics. Therefore, chemical inhibitors can selectively target AgrA in S. aureus to promote host defense while sparing agr signaling in S. epidermidis and limiting resistance development.

• Publisher: Public Library of Science,Public Library of Science (PLoS)
• Subject: Animals / Anti-Bacterial Agents - adverse effects / Anti-Bacterial Agents - chemistry / Anti-Bacterial Agents - pharmacology / Anti-Bacterial Agents - therapeutic use / Antibiotics / Bacteria / Bacterial infections / Bacterial Proteins - antagonists & inhibitors / Bacterial Proteins - chemistry / Bacterial Proteins - genetics / Bacterial Proteins - metabolism / Bacteriology / Cell Line, Transformed / Development and progression / Drug Discovery / Genes, Reporter - drug effects / Genetic aspects / High-Throughput Screening Assays / Immunity, Innate - drug effects / Macrophages - drug effects / Macrophages - immunology / Macrophages - metabolism / Macrophages - microbiology / Male / Medical research / Medicine and Health Sciences / Mice, Hairless / Mice, Knockout / Molecular Conformation / Molecular Docking Simulation / Molecular Targeted Therapy - adverse effects / Mutation / Phagocytosis - drug effects / Physiological aspects / Promoter Regions, Genetic - drug effects / Quinazolinones - adverse effects / Quinazolinones - chemistry / Quinazolinones - pharmacology / Quinazolinones - therapeutic use / Quorum sensing / Quorum Sensing - drug effects / Skin - drug effects / Skin - microbiology / Staphylococcal Skin Infections - drug therapy / Staphylococcal Skin Infections - immunology / Staphylococcal Skin Infections - microbiology / Staphylococcus aureus / Staphylococcus aureus - drug effects / Staphylococcus aureus - growth & development / Staphylococcus aureus - immunology / Staphylococcus aureus - physiology / Staphylococcus aureus infections / Staphylococcus epidermidis - drug effects / Staphylococcus epidermidis - growth & development / Staphylococcus epidermidis - immunology / Staphylococcus epidermidis - physiology / Staphylococcus infections / Trans-Activators - antagonists & inhibitors / Trans-Activators - chemistry / Trans-Activators - genetics / Trans-Activators - metabolism / Triazoles - adverse effects / Triazoles - chemistry / Triazoles - pharmacology / Triazoles - therapeutic use / Virulence (Microbiology)