Journal Article

Design and validation of an oligonucleotide microarray for the detection of genomic rearrangements associated with common hereditary cancer syndromes

Gutin, Natalia, Holladay, Jayson, Roa, Benjamin, Theisen, Aaron, Judkins, Thaddeus, Arnell, Christopher, Burton, Collin, Craft, Jonathan, Woolstenhulme, Nick, Moyes, Kelsey, Ryder, Matthew, Mancini-DiNardo, Debora, Schoenberger, Jeremy, Murray, Adam

2014

Overview

Background Conventional Sanger sequencing reliably detects the majority of genetic mutations associated with hereditary cancers, such as single-base changes and small insertions or deletions. However, detection of genomic rearrangements, such as large deletions and duplications, requires special technologies. Microarray analysis has been successfully used to detect large rearrangements (LRs) in genetic disorders. Methods We designed and validated a high-density oligonucleotide microarray for the detection of gene-level genomic rearrangements associated with hereditary breast and ovarian cancer (HBOC), Lynch, and polyposis syndromes. The microarray consisted of probes corresponding to the exons and flanking introns of BRCA1 and BRCA2 (≈1,700) and Lynch syndrome/polyposis genes MLH1 , MSH2 , MSH6 , APC , MUTYH , and EPCAM (≈2,200). We validated the microarray with 990 samples previously tested for LR status in BRCA1, BRCA2 , MLH1 , MSH2 , MSH6 , APC , MUTYH , or EPCAM . Microarray results were 100% concordant with previous results in the validation studies. Subsequently, clinical microarray analysis was performed on samples from patients with a high likelihood of HBOC mutations (13,124), Lynch syndrome mutations (18,498), and polyposis syndrome mutations (2,739) to determine the proportion of LRs. Results Our results demonstrate that LRs constitute a substantial proportion of genetic mutations found in patients referred for hereditary cancer genetic testing. Conclusion The use of microarray comparative genomic hybridization (CGH) for the detection of LRs is well-suited as an adjunct technology for both single syndrome (by Sanger sequencing analysis) and extended gene panel testing by next generation sequencing analysis. Genetic testing strategies using microarray analysis will help identify additional patients carrying LRs, who are predisposed to various hereditary cancers.

• Publisher: BioMed Central,BioMed Central Ltd,Springer Nature B.V
• Subject: Analysis / Apoptosis / Biomedical and Life Sciences / Biomedicine / Cancer / Cancer Research / Diagnosis / Exons / Genes / Genetic aspects / Genetic research / Genetic screening / Genomics - methods / Humans / Immunology / Instrument industry / Mutation / MutS Homolog 2 Protein - genetics / Neoplastic Syndromes, Hereditary - diagnosis / Neoplastic Syndromes, Hereditary - genetics / Oligonucleotide Array Sequence Analysis - methods / Oncology / Reproducibility of Results / Translocation, Genetic