Journal Article

Lateral root morphogenesis is dependent on the mechanical properties of the overlaying tissues

Lucas, Mikaël, Bennett, Malcolm J, Department of Plant Biotechnology and Genetics [Ghent] ; Universiteit Gent = Ghent University = Université de Gand (UGENT), Barbeau, Daniel, Universität Heidelberg [Heidelberg] = Heidelberg University, Modeling plant morphogenesis at different scales, from genes to phenotype (VIRTUAL PLANTS) ; Centre Inria d'Université Côte d'Azur (CRISAM) ; Institut National de Recherche en Informatique et en Automatique (Inria)-Institut National de Recherche en Informatique et en Automatique (Inria)-Institut National de la Recherche Agronomique (INRA)-Amélioration génétique et adaptation des plantes méditerranéennes et tropicales (UMR AGAP) ; Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)-Centre de Coopér

2013

Overview

In Arabidopsis, lateral root primordia (LRPs) originate from pericycle cells located deep within the parental root and have to emerge through endodermal, cortical, and epidermal tissues. These overlaying tissues place biomechanical constraints on the LRPs that are likely to impact their morphogenesis. This study probes the interplay between the patterns of cell division, organ shape, and overlaying tissues on LRP morphogenesis by exploiting recent advances in live plant cell imaging and image analysis. Our 3D/4D image analysis revealed that early stage LRPs exhibit tangential divisions that create a ring of cells corralling a population of rapidly dividing cells at its center. The patterns of division in the latter population of cells during LRP morphogenesis are not stereotypical. In contrast, statistical analysis demonstrated that the shape of new LRPs is highly conserved. We tested the relative importance of cell division pattern versus overlaying tissues on LRP morphogenesis using mutant and transgenic approaches. The double mutant aurora1 (aur1) aur2 disrupts the pattern of LRP cell divisions and impacts its growth dynamics, yet the new organ's dome shape remains normal. In contrast, manipulating the properties of overlaying tissues disrupted LRP morphogenesis. We conclude that the interaction with overlaying tissues, rather than the precise pattern of divisions, is most important for LRP morphogenesis and optimizes the process of lateral root emergence.

• Publisher: National Academy of Sciences,CCSD,National Acad Sciences
• Subject: Arabidopsis / Arabidopsis - cytology / Arabidopsis - genetics / Arabidopsis - metabolism / Arabidopsis Proteins / Arabidopsis Proteins - genetics / Arabidopsis Proteins - metabolism / Aurora Kinases / Auxins / Biological Sciences / Biomechanics / Cell Division / Cell Division - physiology / Cell lines / Daughter cells / Flowers & plants / genetically modified organisms / image analysis / Imaging / Life Sciences / Mechanical properties / Microscopy / Morphogenesis / mutants / Plant biology / Plant cells / Plant Development / Plant Development - physiology / Plant Roots / Plant Roots - cytology / Plant Roots - genetics / Plant Roots - growth & development / Plant tissues / Plants / Protein Serine-Threonine Kinases - genetics / Protein Serine-Threonine Kinases - metabolism / Protein-Serine-Threonine Kinases / root primordia / Statistical analysis / Vegetal Biology
• ISBN: 0003180319000