Journal Article

Capturing the interactome of newly transcribed RNA

Liu, Yu-lin, Pulido-quetglas, Carlos, Wang, Wei, Zhou, Jiajian, Yang, Yun-gui, Li, Nan, Zhang, Gong, Anko, Minna-liisa, Liu, Xiao, Wang, Huating, Lim, Do-hwan, Bao, Xichen, Kanwal, Shahzina, Shao, Changwei, Ward, Carl, Khan, Muhammad J, Ji, Lu, Zhang, Biliang, Lai, Yiwei, Esteban, Miguel A, Luo, Zhiwei, Yin, Menghui, Li, Na, Yang, Jianhua, He, Minghui, Wang, Yangming, Fu, Xiang-dong, Tariq, Muqddas, Wang, Tong, Jauch, Ralf, Qin, Baoming, Hutchins, Andrew P, Wang, Dongye, Wang, Xiwei, Zhu, Xihua, Chen, Yiwen, Guo, Xiangpeng, Lv, Yuan, Sun, Hao, Zhang, Chaolin

2018

Overview

We combine the labeling of newly transcribed RNAs with 5-ethynyluridine with the characterization of bound proteins. This approach, named capture of the newly transcribed RNA interactome using click chemistry (RICK), systematically captures proteins bound to a wide range of RNAs, including nascent RNAs and traditionally neglected nonpolyadenylated RNAs. RICK has identified mitotic regulators amongst other novel RNA-binding proteins with preferential affinity for nonpolyadenylated RNAs, revealed a link between metabolic enzymes/factors and nascent RNAs, and expanded the known RNA-bound proteome of mouse embryonic stem cells. RICK will facilitate an in-depth interrogation of the total RNA-bound proteome in different cells and systems.

• Publisher: Nature Publishing Group
• Subject: Biotin / Chemical synthesis / Embryo cells / Interrogation / Labeling / Proteins / Proteomes / Regulators / Ribonucleic acid / RNA / RNA-binding protein / Stem cell transplantation / Stem cells