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Culture Supernatants of Lactobacillus gasseri and L. crispatus Inhibit Candida albicans Biofilm Formation and Adhesion to HeLa Cells
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Culture Supernatants of Lactobacillus gasseri and L. crispatus Inhibit Candida albicans Biofilm Formation and Adhesion to HeLa Cells
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Journal Article
Culture Supernatants of Lactobacillus gasseri and L. crispatus Inhibit Candida albicans Biofilm Formation and Adhesion to HeLa Cells
2018
Overview
Purpose
Vulvovaginal candidiasis (VVC) is a common superficial infection of the vaginal mucous membranes caused by the fungus
Candida albicans
. The aim of this study was to assess the mechanisms underlying the inhibitory effects of the culture supernatants of
Lactobacillus gasseri
and
L. crispatus
, the predominant microbiota in Asian healthy women, on
C. albicans
biofilm formation. The inhibition of
C. albicans
adhesion to HeLa cells by
Lactobacillus
culture supernatant was also investigated.
Methods
Candida albicans
biofilm was formed on polystyrene flat-bottomed 96-well plates, and the inhibitory effects on the initial colonization and maturation phases were determined using the XTT reduction assay. The expression levels of biofilm formation-associated genes (
HWP1, ECE1, ALS3, BCR1
,
EFG1
,
TEC1
, and
CPH1
) were determined by reverse transcription quantitative polymerase chain reaction. The inhibition of
C
.
albicans
adhesion to HeLa cells by
Lactobacillus
culture supernatant was evaluated by enumerating viable
C. albicans
cells.
Results
The culture supernatants of both
Lactobacillus
species inhibited the initial colonization and maturation of
C. albicans
biofilm. The expression levels of all biofilm formation-related genes were downregulated in the presence of
Lactobacillus
culture supernatant. The culture supernatant also inhibited
C. albicans
adhesion to HeLa cells.
Conclusion
The culture supernatants of
L
.
gasseri
and
L
.
crispatus
inhibited
C
.
albicans
biofilm formation by downregulating biofilm formation-related genes and
C. albicans
adhesion to HeLa cells. These findings support the notion that
Lactobacillus
metabolites may be useful alternatives to antifungal drugs for the management of VVC.
Publisher
Springer Netherlands,Springer,Springer Nature B.V
Subject
