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Molecular characterization of Mannheimia haemolytica isolates associated with pneumonic cases of sheep in selected areas of Central Ethiopia
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Molecular characterization of Mannheimia haemolytica isolates associated with pneumonic cases of sheep in selected areas of Central Ethiopia
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Molecular characterization of Mannheimia haemolytica isolates associated with pneumonic cases of sheep in selected areas of Central Ethiopia
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Journal Article
Molecular characterization of Mannheimia haemolytica isolates associated with pneumonic cases of sheep in selected areas of Central Ethiopia
2018
Overview
Background
Mannheimia haemolytica
has been recognized as the principal cause of pneumonic pasteurellosis in sheep and goats. It is one of the important diseases of small ruminants in Ethiopia. While annual vaccination using a monovalent vaccine (inactivated
Pasteurella multocida
biotype A) is common, respiratory diseases are still reported in various parts of Ethiopia. This suggests the need for further investigation into the species and strains responsible for the disease, which is vital information for development of a multivalent vaccine. The objective of the current study was to isolate
M. heamolytica
associated with pneumonic cases of sheep in selected areas of Central Ethiopia, determine its role and the strains/genotypes of the bacterium circulating in the study area.
Results
Bacteriological analysis of nasal swab samples collected from a total of 76 pneumonic cases of sheep showed that
M. haemolytica
was isolated from 26 of them while
B.trehalosi
from two cases
.
Further molecular analyses of the isolates using
M. haemolytica
species-specific and
M.haemolytica
serotype-1 antigen specific PCR assays revealed, 26 of the isolates were identified as
M. haemolytica
of which 21 of them were
M. haemolytica
serotype-1. Both
M. haemolytica
and
B.trehalosi
isolates were not detected in a PCR assay targeting capsular biosynthesis gene (
capA
) of
P.multocida
despite the non-specific products observed in
M. haemolytica
isolates. Phylogenetic analysis of
M. haemolytica
isolates included in this study in comparison with the reference strains with respect to
PHSSA
and
Rpt2
genes revealed that the Ethiopian
M. haemolytica
isolates constituted three distinct genotypes consistent with site of origin.
Conclusion
The study indicated that
M.haemolytica
is commonly associated with cases of pneumonia in sheep in the study areas of central Ethiopia although the remaining other pathogens responsible for majority of the cases are yet to be determined. Molecular characterization revealed the existence of three genotypes of
M. haemolytica
circulating in the study areas consistent to the site of isolation. The findings suggest further extensive work to determine all pathogens associated with sheep pneumonia and the strain distribution of
M. heamolytica
to understand its molecular epidemiology at national level and design cost effective prevention and control methods.
