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Oxidative release of natural glycans for functional glycomics
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Oxidative release of natural glycans for functional glycomics
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Journal Article
Oxidative release of natural glycans for functional glycomics
2016
Overview
Treating glycoproteins with household bleach releases N- and O-glycans for further structural probing. Bleach treatment of glycosphingolipids releases glycan nitriles.
Glycans have essential roles in biology and the etiology of many diseases. A major hurdle in studying glycans through functional glycomics is the lack of methods to release glycans from diverse types of biological samples. Here we describe an oxidative strategy using household bleach to release all types of free reducing N-glycans and O-glycan-acids from glycoproteins, and glycan nitriles from glycosphingolipids. Released glycans are directly useful in glycomic analyses and can be derivatized fluorescently for functional glycomics. This chemical method overcomes the limitations in glycan generation and promotes archiving and characterization of human and animal glycomes and their functions.
Publisher
Nature Publishing Group US,Nature Publishing Group
Subject
/ 49/61
/ Analysis
/ Animals
/ Biomedical Engineering/Biotechnology
/ Chickens
/ Chromatography, High Pressure Liquid
/ Etiology
/ Female
/ Gastrointestinal Tract - chemistry
/ Glycosphingolipids - chemistry
/ High performance liquid chromatography
/ Sodium Hypochlorite - chemistry
/ Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
