Journal Article

Construction of genotyping-by-sequencing based high-density genetic maps and QTL mapping for fusarium wilt resistance in pigeonpea

Varshney, R K, Tathineni, R, Sharma, M, Garg, V, Muniswamy, S, Ghanta, A, Saxena, R K, Kale, S M, Singh, V K, Yamini, K N, Sameer Kumar, C V, Rathore, A, Parupalli, S, Kumar, V, Saxena, K B, Kishor, P B K, Das, R R

2017

Overview

Fusarium wilt (FW) is one of the most important biotic stresses causing yield losses in pigeonpea. Genetic improvement of pigeonpea through genomics-assisted breeding (GAB) is an economically feasible option for the development of high yielding FW resistant genotypes. In this context, two recombinant inbred lines (RILs) (ICPB 2049 × ICPL 99050 designated as PRIL_A and ICPL 20096 × ICPL 332 designated as PRIL_B) and one F2 (ICPL 85063 × ICPL 87119) populations were used for the development of high density genetic maps. Genotyping-by-sequencing (GBS) approach was used to identify and genotype SNPs in three mapping populations. As a result, three high density genetic maps with 964, 1101 and 557 SNPs with an average marker distance of 1.16, 0.84 and 2.60 cM were developed in PRIL_A, PRIL_B and F2, respectively. Based on the multi-location and multi-year phenotypic data of FW resistance a total of 14 quantitative trait loci (QTLs) including six major QTLs explaining >10% phenotypic variance explained (PVE) were identified. Comparative analysis across the populations has revealed three important QTLs (qFW11.1, qFW11.2 and qFW11.3) with upto 56.45% PVE for FW resistance. This is the first report of QTL mapping for FW resistance in pigeonpea and identified genomic region could be utilized in GAB.

• Publisher: Nature Publishing Group,Nature Publishing Group UK,Nature Portfolio
• Subject: 45/22 / 45/23 / 631/208/8 / 631/449/711 / Breeding / Cajanus - microbiology / Cajanus cajan / Chromosome Mapping / Comparative analysis / Fusarium - genetics / Gene loci / Gene mapping / Genetic improvement / Genetics, Population / Genome, Fungal / Genomics - methods / Genotypes / Genotyping / High-Throughput Nucleotide Sequencing / Humanities and Social Sciences / Inbreeding / Molecular Typing / multidisciplinary / Phenotype / Polymorphism, Single Nucleotide / Quantitative Trait Loci / Science / Science (multidisciplinary) / Single-nucleotide polymorphism / Wilt