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Imaging of anticancer drug action in single cells
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Journal Article
Imaging of anticancer drug action in single cells
2017
Overview
Key Points
The
in vivo
microscopy toolbox enables extended live-animal 3D imaging in orthotopic disease sites, deeper imaging through tissue and multiple simultaneous molecular measurements.
Imaging of multiple single cells can assess tumour heterogeneity, tumour evolution following drug treatment and drug–target binding; it also enables visualization of micro-anatomical structures and distinct and rare cell types.
Fluorescently labelled drugs and nanoformulations reveal mechanisms of delivery and binding within tumours.
Imaging can show differences in drug response
in vivo
compared with
in vitro
tissue culture models.
Components of the tumour microenvironment, including the extracellular matrix, immune cells and vasculature, can be studied for their role in influencing drug action.
The distribution and effect of immunotherapies including those that block immune checkpoints can be visualized in the tumour and draining lymph nodes.
In vivo
microscopy provides a complementary high-resolution perspective to lower-resolution imaging modalities such as positron emission tomography–computed tomography and magnetic resonance imaging that are used in the clinic.
This Review summarizes developments in the imaging of
in vivo
anticancer drug action, with a focus on microscopy approaches at the single-cell level and translational lessons for the clinic.
Imaging is widely used in anticancer drug development, typically for whole-body tracking of labelled drugs to different organs or to assess drug efficacy through volumetric measurements. However, increasing attention has been drawn to pharmacology at the single-cell level. Diverse cell types, including cancer-associated immune cells, physicochemical features of the tumour microenvironment and heterogeneous cell behaviour all affect drug delivery, response and resistance. This Review summarizes developments in the imaging of
in vivo
anticancer drug action, with a focus on microscopy approaches at the single-cell level and translational lessons for the clinic.
Publisher
Nature Publishing Group UK,Nature Publishing Group
