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Neurochemical and behavioral characterization of neuronal glutamate transporter EAAT3 heterozygous mice
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Neurochemical and behavioral characterization of neuronal glutamate transporter EAAT3 heterozygous mice
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Journal Article
Neurochemical and behavioral characterization of neuronal glutamate transporter EAAT3 heterozygous mice
2017
Overview
Background
Obsessive–compulsive disorder (OCD) is a severe neuropsychiatric condition affecting 1–3% of the worldwide population. OCD has a strong genetic component, and the
SLC1A1
gene that encodes neuronal glutamate transporter EAAT3 is a strong candidate for this disorder. To evaluate the impact of reduced EAAT3 expression in vivo, we studied male EAAT3 heterozygous and wild-type littermate mice using a battery of behavioral paradigms relevant to anxiety (open field test, elevated plus maze) and compulsivity (marble burying), as well as locomotor activity induced by amphetamine. Using high-performance liquid chromatography, we also determined tissue neurotransmitter levels in cortex, striatum and thalamus—brain areas that are relevant to OCD.
Results
Compared to wild-type littermates, EAAT3 heterozygous male mice have unaltered baseline anxiety-like, compulsive-like behavior and locomotor activity. Administration of acute amphetamine (5 mg/kg intraperitoneally) increased locomotion with no differences across genotypes. Tissue levels of glutamate, GABA, dopamine and serotonin did not vary between EAAT3 heterozygous and wild-type mice.
Conclusions
Our results indicate that reduced EAAT3 expression does not impact neurotransmitter content in the corticostriatal circuit nor alter anxiety or compulsive-like behaviors.
Publisher
BioMed Central,BioMed Central Ltd,Sociedad de Biología de Chile,BMC
Subject
/ BIOLOGY
/ Biomedical and Life Sciences
/ EAAT3
/ Excitatory Amino Acid Transporter 3 - genetics
/ Excitatory Amino Acid Transporter 3 - metabolism
/ Genotype
/ Male
/ Mice
/ Neuronal glutamate transporter
/ Neurons
/ Obsessive-compulsive disorder
/ Obsessive-Compulsive Disorder - genetics
/ Obsessive-Compulsive Disorder - metabolism
/ SLC1A1
