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A benzene-degrading nitrate-reducing microbial consortium displays aerobic and anaerobic benzene degradation pathways
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A benzene-degrading nitrate-reducing microbial consortium displays aerobic and anaerobic benzene degradation pathways
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Journal Article
A benzene-degrading nitrate-reducing microbial consortium displays aerobic and anaerobic benzene degradation pathways
2018
Overview
In this study, we report transcription of genes involved in aerobic and anaerobic benzene degradation pathways in a benzene-degrading denitrifying continuous culture. Transcripts associated with the family
Peptococcaceae
dominated all samples (21–36% relative abundance) indicating their key role in the community. We found a highly transcribed gene cluster encoding a presumed anaerobic benzene carboxylase (AbcA and AbcD) and a benzoate-coenzyme A ligase (BzlA). Predicted gene products showed >96% amino acid identity and similar gene order to the corresponding benzene degradation gene cluster described previously, providing further evidence for anaerobic benzene activation via carboxylation. For subsequent benzoyl-CoA dearomatization,
bam
-like genes analogous to the ones found in other strict anaerobes were transcribed, whereas gene transcripts involved in downstream benzoyl-CoA degradation were mostly analogous to the ones described in facultative anaerobes. The concurrent transcription of genes encoding enzymes involved in oxygenase-mediated aerobic benzene degradation suggested oxygen presence in the culture, possibly formed via a recently identified nitric oxide dismutase (Nod). Although we were unable to detect transcription of Nod-encoding genes, addition of nitrite and formate to the continuous culture showed indication for oxygen production. Such an oxygen production would enable aerobic microbes to thrive in oxygen-depleted and nitrate-containing subsurface environments contaminated with hydrocarbons.
Publisher
Nature Publishing Group UK,Nature Publishing Group
Subject
/ 38/91
/ 704/172
/ Benzene
/ Biodegradation, Environmental
/ Biofilms
/ Gene Expression Regulation, Bacterial
/ Genes
/ Humanities and Social Sciences
/ Laboratorium voor Microbiologie
/ Metabolic Networks and Pathways
/ Oxygen
/ Peptococcaceae - growth & development
/ Science
/ Systeem en Synthetische Biologie
/ Systems and Synthetic Biology
/ VLAG
/ WIMEK
