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Single-cell analysis of early B-lymphocyte development suggests independent regulation of lineage specification and commitment in vivo
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Single-cell analysis of early B-lymphocyte development suggests independent regulation of lineage specification and commitment in vivo
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Journal Article
Single-cell analysis of early B-lymphocyte development suggests independent regulation of lineage specification and commitment in vivo
2012
Overview
To better understand the process of B-lymphocyte lineage restriction, we have investigated molecular and functional properties in early B-lineage cells from Pax-5 –deficient animals crossed to a B-lineage–restricted reporter mouse, allowing us to identify B-lineage–specified progenitors independently of conventional surface markers. Pax-5 deficiency resulted in a dramatic increase in the frequency of specified progenitor B-cells marked by expression of a λ5 (Igll1) promoter-controlled reporter gene. Gene expression analysis of ex vivo isolated progenitor cells revealed that Pax-5 deficiency has a minor impact on B-cell specification. However, single-cell in vitro differentiation analysis of ex vivo isolated cells revealed that specified B-lineage progenitors still displayed a high degree of plasticity for development into NK or T lineage cells. In contrast, we were unable to detect any major changes in myeloid lineage potential in specified Pax-5–deficient cells. By comparison of gene expression patterns in ex vivo isolated Pax-5– and Ebf-1–deficient progenitors, it was possible to identify a set of B-cell–restricted genes dependent on Ebf-1 but not Pax-5, supporting the idea that B-cell specification and commitment is controlled by distinct regulatory networks.
Publisher
National Academy of Sciences,National Acad Sciences
Subject
/ Cell Differentiation - physiology
/ Deltex
/ Gene Expression Regulation - immunology
/ Genes
/ Humans
/ Killer Cells, Natural - cytology
/ Killer Cells, Natural - immunology
/ Liver
/ MEDICIN
/ MEDICINE
/ Mice
/ Notch-1
/ PAX5 Transcription Factor - genetics
/ PAX5 Transcription Factor - immunology
/ Proteins
