Journal Article

Effects of tissue degradation by collagenase and elastase on the biaxial mechanics of porcine airways

Ramirez, Gustavo O., Eskandari, Mona, Sattari, Samaneh, Mariano, Crystal A.

2023

Overview

Background Common respiratory illnesses, such as emphysema and chronic obstructive pulmonary disease, are characterized by connective tissue damage and remodeling. Two major fibers govern the mechanics of airway tissue: elastin enables stretch and permits airway recoil, while collagen prevents overextension with stiffer properties. Collagenase and elastase degradation treatments are common avenues for contrasting the role of collagen and elastin in healthy and diseased states; while previous lung studies of collagen and elastin have analyzed parenchymal strips in animal and human specimens, none have focused on the airways to date. Methods Specimens were extracted from the proximal and distal airways, namely the trachea, large bronchi, and small bronchi to facilitate evaluations of material heterogeneity, and subjected to biaxial planar loading in the circumferential and axial directions to assess airway anisotropy. Next, samples were subjected to collagenase and elastase enzymatic treatment and tensile tests were repeated. Airway tissue mechanical properties pre- and post-treatment were comprehensively characterized via measures of initial and ultimate moduli, strain transitions, maximum stress, hysteresis, energy loss, and viscoelasticity to gain insights regarding the specialized role of individual connective tissue fibers and network interactions. Results Enzymatic treatment demonstrated an increase in airway tissue compliance throughout loading and resulted in at least a 50% decrease in maximum stress overall. Strain transition values led to significant anisotropic manifestation post-treatment, where circumferential tissues transitioned at higher strains compared to axial counterparts. Hysteresis values and energy loss decreased after enzymatic treatment, where hysteresis reduced by almost half of the untreated value. Anisotropic ratios exhibited axially led stiffness at low strains which transitioned to circumferentially led stiffness when subjected to higher strains. Viscoelastic stress relaxation was found to be greater in the circumferential direction for bronchial airway regions compared to axial counterparts. Conclusion Targeted fiber treatment resulted in mechanical alterations across the loading range and interactions between elastin and collagen connective tissue networks was observed. Providing novel mechanical characterization of elastase and collagenase treated airways aids our understanding of individual and interconnected fiber roles, ultimately helping to establish a foundation for constructing constitutive models to represent various states and progressions of pulmonary disease.

• Publisher: BioMed Central,BioMed Central Ltd,Nature Publishing Group,BMC
• Subject: Airway tissue mechanics / Analysis / Animals / Anisotropy / Biaxial testing / Biodegradation / Biomechanical Phenomena / Biomechanics / Bronchi / Bronchus / Care and treatment / Cartilage / Chronic obstructive pulmonary disease / Circumferences / Collagen / Collagen - metabolism / Collagenase / Collagenases - metabolism / Collagenases - pharmacology / Connective tissue / Connective tissue diseases / Connective tissues / Constitutive models / Degradation / Diagnosis / Elastase / Elastin / Elastin - metabolism / Emphysema / Energy loss / Fibers / Heterogeneity / Humans / Hysteresis / Load / Lung - metabolism / Lung diseases / Lung diseases, Obstructive / Lungs / Mechanical properties / Mechanics (physics) / Medicine / Medicine & Public Health / Methods / Obstructive lung disease / Pancreatic Elastase / Physiology / Pneumology/Respiratory System / Progressions / Pulmonary biomechanics / Regions / Respiratory diseases / Respiratory tract / Statistical analysis / Stiffness / Strain / Stress relaxation / Swine / Tensile tests / Tissues / Trachea / Viscoelasticity