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Biological cellulose saccharification using a coculture of Clostridium thermocellum and Thermobrachium celere strain A9
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Biological cellulose saccharification using a coculture of Clostridium thermocellum and Thermobrachium celere strain A9
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Journal Article
Biological cellulose saccharification using a coculture of Clostridium thermocellum and Thermobrachium celere strain A9
2022
Overview
An anaerobic thermophilic bacterial strain, A9 (NITE P-03545), that secretes β-glucosidase was newly isolated from wastewater sediments by screening using esculin. The 16S rRNA gene sequence of strain A9 had 100% identity with that of
Thermobrachium celere
type strain JW/YL-NZ35. The complete genome sequence of strain A9 showed 98.4% average nucleotide identity with strain JW/YL-NZ35. However, strain A9 had different physiological properties from strain JW/YL-NZ35, which cannot secrete β-glucosidases or grow on cellobiose as the sole carbon source. The key β-glucosidase gene (
TcBG1
) of strain A9, which belongs to glycoside hydrolase family 1, was characterized. Recombinant β-glucosidase (rTcBG1) hydrolyzed cellooligosaccharides to glucose effectively. Furthermore, rTcBG1 showed high thermostability (at 60°C for 2 days) and high glucose tolerance (IC
50
= 0.75 M glucose), suggesting that rTcBG1 could be used for biological cellulose saccharification in cocultures with
Clostridium thermocellum
. High cellulose degradation was observed when strain A9 was cocultured with
C. thermocellum
in a medium containing 50 g/l crystalline cellulose, and glucose accumulation in the culture supernatant reached 35.2 g/l. In contrast, neither a monoculture of
C. thermocellum
nor coculture of
C. thermocellum
with strain JW/YL-NZ35 realized efficient cellulose degradation or high glucose accumulation. These results show that the β-glucosidase secreted by strain A9 degrades cellulose effectively in combination with
C. thermocellum
cellulosomes and has the potential to be used in a new biological cellulose saccharification process that does not require supplementation with β-glucosidases.
Key points
•
Strain A9 can secrete a thermostable β-glucosidase that has high glucose tolerance
•
A coculture of strain A9 and C. thermocellum showed high cellulose degradation
•
Strain A9 achieves biological saccharification without addition of β-glucosidase
Publisher
Springer Berlin Heidelberg,Springer,Springer Nature B.V
Subject
/ Analysis
/ Applied Microbial and Cell Physiology
/ beta-Glucosidase - metabolism
/ Biomedical and Life Sciences
/ carbon
/ Clostridium thermocellum - genetics
/ Clostridium thermocellum - metabolism
/ Esculin
/ genes
/ Genomes
/ Genomics
/ Glucose
/ Identification and classification
/ Methods
/ Microbial Genetics and Genomics
/ RNA, Ribosomal, 16S - genetics
/ RNA, Ribosomal, 16S - metabolism
/ rRNA 16S
