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Kit-based synthesis of 2-deoxy-2-18F-fluoro-d-sorbitol for bacterial imaging
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Kit-based synthesis of 2-deoxy-2-18F-fluoro-d-sorbitol for bacterial imaging
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Journal Article
Kit-based synthesis of 2-deoxy-2-18F-fluoro-d-sorbitol for bacterial imaging
2021
Overview
Clinically available imaging tools for diagnosing infections rely on structural changes in the affected tissues. They therefore lack specificity and cannot differentiate between oncologic, inflammatory and infectious processes. We have developed 2-deoxy-2-[
18
F]fluoro-
d
-sorbitol (
18
F-FDS) as an imaging agent to visualize infections caused by Enterobacterales, which represent the largest group of bacterial pathogens in humans and are responsible for severe infections, often resulting in sepsis or death. A clinical study in 26 prospectively enrolled patients demonstrated that
18
F-FDS positron emission tomography (PET) was safe, and could detect and localize infections due to drug-susceptible or multi-drug-resistant Enterobacterales strains as well as differentiate them from other pathologies (sterile inflammation or cancer).
18
F-FDS is cleared almost exclusively through renal filtration and has also shown potential as a PET agent for functional renal imaging. Since most PET radionuclides have a short half-life, maximal clinical impact will require fast, on-demand synthesis with limited infrastructure and personnel. To meet this demand, we developed a kit-based solid phase method that uses commercially and widely available 2-deoxy-2-[
18
F]fluoro-
d
-glucose as the precursor and allows
18
F-FDS to be produced and purified in one step at room temperature. The
18
F-FDS kit consists of a solid-phase extraction cartridge packed with solid supported borohydride (MP-borohydride), which can be attached to a second cartridge to reduce pH. We evaluated the effects of different solid supported borohydride reagents, cartridge size, starting radioactivity, volumes and flow rates in the radiochemical yield and purity. The optimized protocol can be completed in <30 min and allows the synthesis of
18
F-FDS in >70% radiochemical yield and >90% radiochemical purity.
Mota et al. describe a protocol for the rapid, room-temperature, kit-based synthesis of
18
F-FDS for positron emission tomography imaging.
Publisher
Nature Publishing Group UK,Nature Publishing Group
Subject
/ Biomedical and Life Sciences
/ Computational Biology/Bioinformatics
/ Fluorine Radioisotopes - administration & dosage
/ Fluorine Radioisotopes - chemistry
/ Glucose
/ Humans
/ Methods
/ Positron emission tomography
/ Positron-Emission Tomography - methods
/ Protocol
/ Purity
/ Radiopharmaceuticals - chemical synthesis
/ Radiopharmaceuticals - chemistry
/ Reagents
/ Sepsis
/ Solid Phase Extraction - methods
/ Sorbitol
/ Sorbitol - administration & dosage
/ Sorbitol - analogs & derivatives
