Journal Article

A novel class of tsRNA signatures as biomarkers for diagnosis and prognosis of pancreatic cancer

Yang, Ping, Chen, Xi, Wang, Yanbo, Jin, Fangfang, Yang, Liuqing, Zhan, Shoubin, Wang, Weixiang, Yuan, Na, Ma, Tonghui

2021

Overview

Results and discussion Ectopic tsRNA signatures in pancreatic cancer serum To explore whether serum tsRNAs could serve as novel biomarkers for PC, we performed preliminary screening by small RNA sequencing followed by qRT-PCR validation on individual basis. Elevated serum tRF-Pro-AGG-004 and tRF-Leu-CAG-002 originate from tumor cells To determine whether the high levels of the two tsRNAs in PC serum were induced by perturbations in PC tissues, we first examined the tissue and paired serum levels of tRF-Pro-AGG-004 and tRF-Leu-CAG-002 from the same patients in a independent clinical cohort (n = 20, Table S4) and observed an positive correlation (Fig. 2A). [...]treatment by two tsRNAs inhibitors in cells showed decreased tsRNAs levels in culture medium (Fig. 2C), suggesting that tsRNAs in culture medium were directly released from PC cells. Traditional pathological staging systems may have reached their limit for predicting outcomes, and new molecular biomarkers may add values. [...]we checked and scored the two-tsRNAs expression in 2 clinical PC cohorts by ISH analysis in standard process.

• Publisher: BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC
• Subject: Animals / Biological markers / Biomarker / Biomarkers / Biomarkers, Tumor / Biomedical and Life Sciences / Biomedicine / Cancer Research / Cell culture / Circulating MicroRNA / Culture media / Disease Models, Animal / Ectopic Gene Expression / Gene Expression Profiling / Health aspects / Heterografts / Hospitals / Humans / Immunohistochemistry / Letter to the Editor / Medical prognosis / Medical screening / Mice / Oncology / Pancreatic cancer / Pancreatic Neoplasms - blood / Pancreatic Neoplasms - diagnosis / Pancreatic Neoplasms - genetics / Pheochromocytoma cells / Prognosis / RNA, Small Untranslated - blood / RNA, Small Untranslated - genetics / RNA, Transfer - blood / RNA, Transfer - genetics / ROC Curve / Serum levels / Signatures / Transcriptome / Transfer RNA / Trinucleotide repeats / tsRNA / Tumor cells