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Integrated application of multi-omics provides insights into cold stress responses in pufferfish Takifugu fasciatus
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Integrated application of multi-omics provides insights into cold stress responses in pufferfish Takifugu fasciatus
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Journal Article
Integrated application of multi-omics provides insights into cold stress responses in pufferfish Takifugu fasciatus
2019
Overview
Background
T. fasciatus
(
Takifugu fasciatus
) faces the same problem as most warm water fish: the water temperature falls far below the optimal growth temperature in winter, causing a massive death of
T. fasciatus
and large economic losses. Understanding of the cold-tolerance mechanisms of this species is still limited. Integrated application of multi-omics research can provide a wealth of information to help us improve our understanding of low-temperature tolerance in fish.
Results
To gain a comprehensive and unbiased molecular understanding of cold-tolerance in
T. fasciatus
, we characterized mRNA-seq and metabolomics of
T. fasciatus
livers using Illumina HiSeq 2500 and UHPLC-Q-TOF MS. We identified 2544 up-regulated and 2622 down-regulated genes in the liver of
T. fasciatus
. A total of 40 differential metabolites were identified, including 9 down-regulated and 31 up-regulated metabolites. In combination with previous studies on proteomics, we have established an mRNA-protein-metabolite interaction network. There are 17 DEMs (differentially-expressed metabolites) and 14 DEGs-DEPs (differentially co-expressed genes and proteins) in the interaction network that are mainly involved in fatty acids metabolism, membrane transport, signal transduction, and DNA damage and defense. We then validated a number of genes in the interaction network by qRT-PCR. Additionally, a number of SNPs (single nucleotide polymorphisms) were revealed through the transcriptome data. These results provide key information for further understanding of the molecular mechanisms of
T. fasciatus
under cold stress.
Conclusion
The data generated by integrated application of multi-omics can facilitate our understanding of the molecular mechanisms of fish response to low temperature stress. We have not only identified potential genes and SNPs involved in cold tolerance, but also show that some nutrient metabolites may be added to the diet to help the overwintering of
T. fasciatus
.
Publisher
BioMed Central,BioMed Central Ltd,BMC
Subject
/ Animal Genetics and Genomics
/ Animals
/ Behavior
/ Biomedical and Life Sciences
/ Cellular signal transduction
/ Cold-Shock Response - genetics
/ Death
/ DNA
/ Fishes
/ Genes
/ High-Throughput Nucleotide Sequencing
/ Liver
/ Microbial Genetics and Genomics
/ Polymorphism, Single Nucleotide
/ Proteins
/ RNA
