Journal Article

Impact of diet in shaping gut microbiota revealed by a comparative study in children from Europe and rural Africa

Massart, Sebastien, Collini, Silvia, De Filippo, Carlotta, Ramazzotti, Matteo, Poullet, Jean Baptiste, Pieraccini, Giuseppe, Hartl, Daniel L., Di Paola, Monica, Lionetti, Paolo, Cavalieri, Duccio

2010

Overview

Gut microbial composition depends on different dietary habits just as health depends on microbial metabolism, but the association of microbiota with different diets in human populations has not yet been shown. In this work, we compared the fecal microbiota of European children (EU) and that of children from a rural African village of Burkina Faso (BF), where the diet, high in fiber content, is similar to that of early human settlements at the time of the birth of agriculture. By using high-throughput 16S rDNA sequencing and biochemical analyses, we found significant differences in gut microbiota between the two groups. BF children showed a significant enrichment in Bacteroidetes and depletion in Firmicutes (P < 0.001), with a unique abundance of bacteria from the genus Prevotella and Xylanibacter, known to contain a set of bacterial genes for cellulose and xylan hydrolysis, completely lacking in the EU children. In addition, we found significantly more short-chain fatty acids (P < 0.001) in BF than in EU children. Also, Enterobacteriaceae (Shigella and Escherichia) were significantly underrepresented in BF than in EU children (P < 0.05). We hypothesize that gut microbiota coevolved with the polysaccharide-rich diet of BF individuals, allowing them to maximize energy intake from fibers while also protecting them from inflammations and noninfectious colonic diseases. This study investigates and compares human intestinal microbiota from children characterized by a modern western diet and a rural diet, indicating the importance of preserving this treasure of microbial diversity from ancient rural communities worldwide.

• Publisher: National Academy of Sciences,National Acad Sciences
• Subject: Agriculture / Bacteria / Bacteria - classification / Bacteria - genetics / Bacteria - growth & development / Bacteroidetes / Biochemical analysis / Biodiversity / Biological Sciences / Birth / Burkina Faso / Cellulose / Child / Child, Preschool / Children / Childrens health / Cluster Analysis / coevolution / Comparative analysis / Comparative studies / Deoxyribonucleic acid / Diet / Diets / digestive system diseases / Digestive tract / DNA / eating habits / Energy intake / Enterobacteriaceae / Escherichia / Europe / European Union / Fatty acids / Fecal coliforms / Feces - microbiology / Feeding Behavior / Female / fiber content / Fibers / Firmicutes / Gastrointestinal Tract - microbiology / Genera / genes / human population / Human populations / Human settlements / Humans / Hydrolysis / Impact analysis / Infant / Inflammation / Intestinal microflora / intestinal microorganisms / Italy / Life sciences / Male / Metabolism / Metagenome - genetics / Metagenomics / Microbiologie / Microbiology / Microbiota / Microorganisms / Phylogeny / Prevotella / ribosomal DNA / RNA, Ribosomal, 16S - genetics / rRNA 16S / Rural areas / rural communities / Rural Population - statistics & numerical data / Sciences du vivant / Sequence Analysis, DNA / Shigella / short chain fatty acids / Urban Population - statistics & numerical data / villages / Western diets / Xylan