Journal Article

Genome-wide association studies for diabetic macular edema and proliferative diabetic retinopathy

Graham, Patricia S., Chang, John H., Hykin, Philip G., Abhary, Sotoodeh, Hewitt, Alex W., Essex, Rohan W., Lake, Stewart R., Jenkins, Alicia J., Gillies, Mark C., Daniell, Mark, Brown, Matthew A., Craig, Jamie E., Pal, Bishwanath, Kaidonis, Georgia, Lamoureux, Ecosse L., Petrovsky, Nikolai, Burdon, Kathryn P.

2018

Overview

Background Diabetic macular edema (DME) and proliferative diabetic retinopathy (PDR) are sight-threatening complications of diabetes mellitus and leading causes of adult-onset blindness worldwide. Genetic risk factors for diabetic retinopathy (DR) have been described previously, but have been difficult to replicate between studies, which have often used composite phenotypes and been conducted in different populations. This study aims to identify genetic risk factors for DME and PDR as separate complications in Australians of European descent with type 2 diabetes. Methods Caucasian Australians with type 2 diabetes were evaluated in a genome-wide association study (GWAS) to compare 270 DME cases and 176 PDR cases with 435 non-retinopathy controls. All participants were genotyped by SNP array and after data cleaning, cases were compared to controls using logistic regression adjusting for relevant covariates. Results The top ranked SNP for DME was rs1990145 ( p  = 4.10 × 10 − 6 , OR = 2.02 95%CI [1.50, 2.72]) on chromosome 2. The top-ranked SNP for PDR was rs918519 ( p  = 3.87 × 10 − 6 , OR = 0.35 95%CI [0.22, 0.54]) on chromosome 5. A trend towards association was also detected at two SNPs reported in the only other reported GWAS of DR in Caucasians; rs12267418 near MALRD1 ( p  = 0.008) in the DME cohort and rs16999051 in the diabetes gene PCSK2 ( p  = 0.007) in the PDR cohort. Conclusion This study has identified loci of interest for DME and PDR, two common ocular complications of diabetes. These findings require replication in other Caucasian cohorts with type 2 diabetes and larger cohorts will be required to identify genetic loci with statistical confidence. There is considerable overlap in the patient cohorts with each retinopathy subtype, complicating the search for genes that contribute to PDR and DME biology.

• Publisher: BioMed Central,BioMed Central Ltd,BMC
• Subject: Biomedical and Life Sciences / Biomedicine / Cytogenetics / Diabetes complications / Diabetic retinopathy / Gene Function / Genetic aspects / Genetic epidemiology and genetic associations / Genetic research / Genetics / Genome-wide association study / Human Genetics / Macular edema / Medical research / Research Article / Type 2 diabetes