Journal Article

Integrated analyses of ionomics, phytohormone profiles, transcriptomics, and metabolomics reveal a pivotal role of carbon-nano sol in promoting the growth of tobacco plants

Liang, Taibo, Zheng, Xueao, Zheng, Qingxia, Hua, Yingpeng, Liu, Pingping, Cao, Peijian, Guo, Yadi, Wang, Lin, Wang, Chen, Xu, Yalong, Chen, Qiansi, Kang, Zhengzhong

2024

Overview

Background Carbon nano sol (CNS) can markedly affect the plant growth and development. However, few systematic analyses have been conducted on the underlying regulatory mechanisms in plants, including tobacco ( Nicotiana tabacum L.). Results Integrated analyses of phenome, ionome, transcriptome, and metabolome were performed in this study to elucidate the physiological and molecular mechanisms underlying the CNS-promoting growth of tobacco plants. We found that 0.3% CNS, facilitating the shoot and root growth of tobacco plants, significantly increased shoot potassium concentrations. Antioxidant, metabolite, and phytohormone profiles showed that 0.3% CNS obviously reduced reactive oxygen species production and increased antioxidant enzyme activity and auxin accumulation. Comparative transcriptomics revealed that the GO and KEGG terms involving responses to oxidative stress, DNA binding, and photosynthesis were highly enriched in response to exogenous CNS application. Differential expression profiling showed that NtNPF7.3/NtNRT1.5 , potentially involved in potassium/auxin transport, was significantly upregulated under the 0.3% CNS treatment. High-resolution metabolic fingerprints showed that 141 and 163 metabolites, some of which were proposed as growth regulators, were differentially accumulated in the roots and shoots under the 0.3% CNS treatment, respectively. Conclusions Taken together, this study revealed the physiological and molecular mechanism underlying CNS-mediated growth promotion in tobacco plants, and these findings provide potential support for improving plant growth through the use of CNS.

• Publisher: BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC
• Subject: Abiotic stress tolerance in plants / Agricultural production / Agriculture / Amino acids / Analysis / antioxidant enzymes / Antioxidants / Auxin / Auxins / Biomedical and Life Sciences / Carbon / Carbon - metabolism / Carbon-nano sol / DNA / Environmental aspects / Enzymatic activity / Enzyme activity / Enzymes / Gene Expression Profiling / gene expression regulation / Growth / growth promotion / Growth regulators / ionome / ionomics / Life Sciences / Metabolism / Metabolites / Metabolome / Metabolomics / Molecular modelling / Nanomaterials / Nanoparticles / Nicotiana - genetics / Nicotiana - growth & development / Nicotiana - metabolism / Nicotiana tabacum / Nitrate allocation / Nitrogen / Oxidative stress / Photosynthesis / Physiological aspects / Physiology / Phytohormones / Plant growth / Plant Growth Regulators - metabolism / Plant hormones / Plant Roots - genetics / Plant Roots - growth & development / Plant Roots - metabolism / Plant Sciences / Plant Shoots - genetics / Plant Shoots - growth & development / Plant Shoots - metabolism / Potassium / Production management / Reactive oxygen species / Regulatory mechanisms (biology) / root growth / Seeds / Shoots / Statistics / Sustainable development / Tobacco / Tobacco (Plant) / Transcriptome / Transcriptomes / Transcriptomics / Tree Biology