Journal Article

Insulin and IGF-1 receptors regulate complex I–dependent mitochondrial bioenergetics and supercomplexes via FoxOs in muscle

Jena, Jayashree, Morales, Pablo E., Abel, E. Dale, Foster, Collin, Poro, Kennedy, Souvenir, Rhonda, Suarez Beltran, Pablo A., O’Neill, Brian T., Bravo-Sagua, Roberto, Bhardwaj, Gourav, Lira, Vitor A., Hinton, Antentor O., Penniman, Christie M., Fuqua, Jordan D., Sivitz, William I., Junck, Taylor L.

2021

Overview

Decreased skeletal muscle strength and mitochondrial dysfunction are characteristic of diabetes. The actions of insulin and IGF-1 through the insulin receptor (IR) and IGF-1 receptor (IGF1R) maintain muscle mass via suppression of forkhead box O (FoxO) transcription factors, but whether FoxO activation coordinates atrophy in concert with mitochondrial dysfunction is unknown. We show that mitochondrial respiration and complex I activity were decreased in streptozotocin (STZ) diabetic muscle, but these defects were reversed in muscle-specific FoxO1, -3, and -4 triple-KO (M-FoxO TKO) mice rendered diabetic with STZ. In the absence of systemic glucose or lipid abnormalities, muscle-specific IR KO (M-IR-/-) or combined IR/IGF1R KO (MIGIRKO) impaired mitochondrial respiration, decreased ATP production, and increased ROS. These mitochondrial abnormalities were not present in muscle-specific IR, IGF1R, and FoxO1, -3, and -4 quintuple-KO mice (M-QKO). Acute tamoxifen-inducible deletion of IR and IGF1R also decreased muscle pyruvate respiration, complex I activity, and supercomplex assembly. Although autophagy was increased when IR and IGF1R were deleted in muscle, mitophagy was not increased. Mechanistically, RNA-Seq revealed that complex I core subunits were decreased in STZ-diabetic and MIGIRKO muscle, and these changes were not present with FoxO KO in STZ-FoxO TKO and M-QKO mice. Thus, insulin-deficient diabetes or loss of insulin/IGF-1 action in muscle decreases complex I-driven mitochondrial respiration and supercomplex assembly in part by FoxO-mediated repression of complex I subunit expression.

• Publisher: American Society for Clinical Investigation
• Subject: Animals / Diabetes Mellitus, Experimental - metabolism / Electron Transport Complex I - metabolism / Energy Metabolism / Forkhead Transcription Factors - deficiency / Forkhead Transcription Factors - genetics / Forkhead Transcription Factors - metabolism / Male / Mice / Mice, Knockout / Mitochondria, Muscle - metabolism / Models, Biological / Muscle, Skeletal - metabolism / Receptor, IGF Type 1 - deficiency / Receptor, IGF Type 1 - genetics / Receptor, IGF Type 1 - metabolism / Receptor, Insulin - deficiency / Receptor, Insulin - genetics / Receptor, Insulin - metabolism