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PSMD8 can serve as potential biomarker and therapeutic target of the PSMD family in ovarian cancer: based on bioinformatics analysis and in vitro validation
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PSMD8 can serve as potential biomarker and therapeutic target of the PSMD family in ovarian cancer: based on bioinformatics analysis and in vitro validation
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PSMD8 can serve as potential biomarker and therapeutic target of the PSMD family in ovarian cancer: based on bioinformatics analysis and in vitro validation
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Journal Article
PSMD8 can serve as potential biomarker and therapeutic target of the PSMD family in ovarian cancer: based on bioinformatics analysis and in vitro validation
2023
Overview
Background
The ubiquity-proteasome system is an indispensable mechanism for regulating intracellular protein degradation, thereby affecting human antigen processing, signal transduction, and cell cycle regulation. We used bioinformatics database to predict the expression and related roles of all members of the
PSMD
family in ovarian cancer. Our findings may provide a theoretical basis for early diagnosis, prognostic assessment, and targeted therapy of ovarian cancer.
Methods
GEPIA, cBioPortal, and Kaplan–Meier Plotter databases were used to analyze the mRNA expression levels, gene variation, and prognostic value of
PSMD
family members in ovarian cancer.
PSMD8
was identified as the member with the best prognostic value. The TISIDB database was used to analyze the correlation between
PSMD8
and immunity, and the role of PSMD8 in ovarian cancer tissue was verified by immunohistochemical experiments. The relationship of PSMD8 expression with clinicopathological parameters and survival outcomes of ovarian cancer patients was analyzed. The effects of PSMD8 on malignant biological behaviors of invasion, migration, and proliferation of ovarian cancer cells were studied by in vitro experiments.
Results
The expression levels of
PSMD8/14
mRNA in ovarian cancer tissues were significantly higher than those in normal ovarian tissues, and the expression levels of
PSMD2/3/4/5/8/11/12/14
mRNA were associated with prognosis. Up-regulation of
PSMD4/8/14
mRNA expression was associated with poor OS, and the up-regulation of
PSMD2/3/5/8
mRNA expression was associated with poor PFS in patients with ovarian serous carcinomas. Gene function and enrichment analysis showed that
PSMD8
is mainly involved in biological processes such as energy metabolism, DNA replication, and protein synthesis. Immunohistochemical experiments showed that PSMD8 was mainly expressed in the cytoplasm and the expression level was correlated with FIGO stage. Patients with high PSMD8 expression had poor prognosis. Overexpression of PSMD8 significantly enhanced the proliferation, migration, and invasion abilities in ovarian cancer cells.
Conclusion
We observed different degrees of abnormal expression of members of
PSMD
family in ovarian cancer. Among these, PSMD8 was significantly overexpressed in ovarian malignant tissue, and was associated with poor prognosis.
PSMDs
, especially
PSMD8
, can serve as potential diagnostic and prognostic biomarkers and therapeutic targets in ovarian cancer.
Publisher
BioMed Central,BioMed Central Ltd,BMC
