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A DNA-based molecular probe for optically reporting cellular traction forces

by Anthony, Peter C , Dumelin, Christoph E , Chen, Christopher S , Trappmann, Britta , Choi, Colin K , Duesterberg, Van K , Liu, David R , Baker, Brendon M , Block, Steven M , Blakely, Brandon L , McGregor, Lynn M

in 14/10 / 631/1647/2204/2112 / 631/1647/245/2225 / 631/61/54 / 631/80/79 / Animals / Bioinformatics / Biological Microscopy / Biological Techniques / Biomedical Engineering/Biotechnology / brief-communication / Cell adhesion / Cell Adhesion - physiology / Cell research / Cells, Cultured / Deoxyribonucleic acid / DNA / DNA Probes - chemistry / Embryo, Mammalian - cytology / Embryo, Mammalian - metabolism / Fibroblasts - cytology / Fibroblasts - metabolism / Focal Adhesions - physiology / Life Sciences / Mechanotransduction, Cellular - physiology / Mice / Microscopy, Fluorescence / Molecular biology / Molecular probes / Probes / Properties / Proteomics / Studies / Traction

2014

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A DNA-based molecular probe for optically reporting cellular traction forces

2014

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2014

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Journal Article

A DNA-based molecular probe for optically reporting cellular traction forces

Anthony, Peter C,

Dumelin, Christoph E,

Chen, Christopher S,

Trappmann, Britta,

Choi, Colin K,

Duesterberg, Van K,

Liu, David R,

Baker, Brendon M,

Block, Steven M,

Blakely, Brandon L,

McGregor, Lynn M

2014

Overview

DNA hairpins with a conjugated fluorophore-quencher pair are attached to surfaces to monitor cellular traction forces at high spatial resolution. We developed molecular tension probes (TPs) that report traction forces of adherent cells with high spatial resolution, can in principle be linked to virtually any surface, and obviate monitoring deformations of elastic substrates. TPs consist of DNA hairpins conjugated to fluorophore-quencher pairs that unfold and fluoresce when subjected to specific forces. We applied TPs to reveal that cellular traction forces are heterogeneous within focal adhesions and localized at their distal edges.

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Publisher

Nature Publishing Group US,Nature Publishing Group

Subject

/ Animals