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Label-Free Biomedical Imaging with High Sensitivity by Stimulated Raman Scattering Microscopy
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Label-Free Biomedical Imaging with High Sensitivity by Stimulated Raman Scattering Microscopy
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Journal Article
Label-Free Biomedical Imaging with High Sensitivity by Stimulated Raman Scattering Microscopy
2008
Overview
Label-free chemical contrast is highly desirable in biomedical imaging. Spontaneous Raman microscopy provides specific vibrational signatures of chemical bonds, but is often hindered by low sensitivity. Here we report a three-dimensional multiphoton vibrational imaging technique based on stimulated Raman scattering (SRS). The sensitivity of SRS imaging is significantly greater than that of spontaneous Raman microscopy, which is achieved by implementing high-frequency (megahertz) phase-sensitive detection. SRS microscopy has a major advantage over previous coherent Raman techniques in that it offers background-free and readily interpretable chemical contrast. We show a variety of biomedical applications, such as differentiating distributions of omega-3 fatty acids and saturated lipids in living cells, imaging of brain and skin tissues based on intrinsic lipid contrast, and monitoring drug delivery through the epidermis.
Publisher
American Association for the Advancement of Science,The American Association for the Advancement of Science
Subject
/ Biological and medical sciences
/ brain
/ Dimethyl Sulfoxide - administration & dosage
/ Dimethyl Sulfoxide - pharmacokinetics
/ drugs
/ Eicosapentaenoic Acid - metabolism
/ Humans
/ Imaging
/ Imaging, Three-Dimensional - methods
/ Investigative techniques, diagnostic techniques (general aspects)
/ Lipids
/ Mice
/ Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques
/ Pumps
/ Skin
/ tissues
/ Tretinoin - administration & dosage
