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Homeostatic regulation of supercoiling sensitivity coordinates transcription of the bacterial genome
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Homeostatic regulation of supercoiling sensitivity coordinates transcription of the bacterial genome
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Homeostatic regulation of supercoiling sensitivity coordinates transcription of the bacterial genome
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Journal Article
Homeostatic regulation of supercoiling sensitivity coordinates transcription of the bacterial genome
2006
Overview
Regulation of cellular growth implies spatiotemporally coordinated programmes of gene transcription. A central question, therefore, is how global transcription is coordinated in the genome. The growth of the unicellular organism
Escherichia coli
is associated with changes in both the global superhelicity modulated by cellular topoisomerase activity and the relative proportions of the abundant DNA‐architectural chromatin proteins. Using a DNA‐microarray‐based approach that combines mutations in the genes of two important chromatin proteins with induced changes of DNA superhelicity, we demonstrate that genomic transcription is tightly associated with the spatial distribution of supercoiling sensitivity, which in turn depends on chromatin proteins. We further demonstrate that essential metabolic pathways involved in the maintenance of growth respond distinctly to changes of superhelicity. We infer that a homeostatic mechanism organizing the supercoiling sensitivity is coordinating the growth‐phase‐dependent transcription of the genome.
Publisher
John Wiley & Sons, Ltd,Nature Publishing Group UK,Springer Nature B.V,EMBO Press
Subject
/ Bacterial Proteins - genetics
/ Bacterial Proteins - metabolism
/ Biochemistry, Molecular Biology
/ Cells
/ DNA
/ DNA, Superhelical - chemistry
/ DNA, Superhelical - genetics
/ DNA, Superhelical - metabolism
/ DNA-Binding Proteins - genetics
/ DNA-Binding Proteins - metabolism
/ E coli
/ Escherichia coli - growth & development
/ Escherichia coli - metabolism
/ Escherichia coli Proteins - genetics
/ Escherichia coli Proteins - metabolism
/ Factor For Inversion Stimulation Protein
/ FIS
/ Genomics
/ H-NS
/ Microbiology and Parasitology
/ Mutation
/ Oligonucleotide Array Sequence Analysis
/ Proteins
/ Transcription Factors - genetics
