Journal Article

OsRCI-1-Mediated GLVs Enhance Rice Resistance to Brown Planthoppers

Xue, Wenhua, Mao, Kaiming, Zhou, Guoxin, Li, Chengzhe, Zhai, Huacai, Lou, Yonggen, Wang, Yuying

2024

Overview

Green leaf volatiles (GLVs) play pivotal roles in plant anti-herbivore defense. This study investigated whether the rice 13-lipoxygense gene OsRCI-1 is involved in GLV production and plant defense in rice. The overexpression of OsRCI-1 (oeRCI lines) in rice resulted in increased wound-induced levels of two prominent GLVs, cis-3-hexen-1-ol and cis-3-hexenal. In a previous study, we found that the overexpression of OsRCI-1 reduced the colonization by the rice brown planthopper (BPH, Nilaparvata lugens) but increased the attractiveness to the egg parasitoid Anagrus nilaparvatae compared to wild-type (WT) plants. This study found that when cis-3-hexen-1-ol, but not cis-3-hexenal, was added to WT plants, it could change the BPH’s colonization preference, i.e., more BPHs preferred to colonize the oeRCI lines. The exogenous application of cis-3-hexen-1-ol or cis-3-hexenal to BPH-infested WT plants could weaken or overturn the preference of A. nilaparvatae for oeRCI lines. However, field experiments revealed that only cis-3-hexenal was attractive to the parasitoid and increased the parasitism rates of BPH eggs. These results indicate that OsRCI-1 is involved in rice GLV production and therefore modulates both direct and indirect defense in rice.

• Publisher: MDPI AG,MDPI
• Subject: Allelochemicals / Anagrus nilaparvatae / Biogenic volatile organic compounds / cis-3-hexen-1-ol / cis-3-hexenal / Cloning / Colonization / Control / Crop production / Eggs / Environmental aspects / Fatty acids / Field tests / Food and nutrition / Genes / Genetic aspects / green leaf volatiles (GLVs) / Herbivores / Hex-3-enal / Identification and classification / Nilaparvata lugens / Parasitism / Physiological aspects / Plant defenses / Plant resistance / Planthoppers / Preferences / Rice / Volatile compounds