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Homogeneous detection of unamplified genomic DNA sequences based on colorimetric scatter of gold nanoparticle probes
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Homogeneous detection of unamplified genomic DNA sequences based on colorimetric scatter of gold nanoparticle probes
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Journal Article
Homogeneous detection of unamplified genomic DNA sequences based on colorimetric scatter of gold nanoparticle probes
2004
Overview
Nucleic acid diagnostics is dominated by fluorescence-based assays that use complex and expensive enzyme-based target or signal-amplification procedures
1
,
2
,
3
,
4
,
5
,
6
. Many clinical diagnostic applications will require simpler, inexpensive assays that can be done in a screening mode. We have developed a 'spot-and-read' colorimetric detection method for identifying nucleic acid sequences based on the distance-dependent optical properties of gold nanoparticles. In this assay, nucleic acid targets are recognized by DNA-modified gold probes, which undergo a color change that is visually detectable when the solutions are spotted onto an illuminated glass waveguide. This scatter-based method enables detection of zeptomole quantities of nucleic acid targets without target or signal amplification when coupled to an improved hybridization method that facilitates probe-target binding in a homogeneous format. In comparison to a previously reported absorbance-based method
7
, this method increases detection sensitivity by over four orders of magnitude. We have applied this method to the rapid detection of
mecA
in methicillin-resistant
Staphylococcus aureus
genomic DNA samples.
Publisher
Nature Publishing Group US,Nature,Nature Publishing Group
Subject
/ Bacterial Proteins - analysis
/ Bacterial Proteins - chemistry
/ Bacterial Proteins - genetics
/ Biological and medical sciences
/ Biomedical and Life Sciences
/ Biomedical Engineering/Biotechnology
/ DNA
/ Fundamental and applied biological sciences. Psychology
/ Genomics
/ letter
/ Methods. Procedures. Technologies
/ Molecular and cellular biology
/ Others
/ Probes
/ Sequence Analysis, DNA - methods
/ Staphylococcus aureus - chemistry
