Journal Article

The role of vascular endothelial growth factor and matrix metalloproteinases in canine lymphoma: in vivo and in vitro study

Essler, Sabine E, Riondato, Fulvio, Aricò, Arianna, Dacasto, Mauro, Aresu, Luca, Castagnaro, Massimo, Rütgen, Barbara C, Gelain, Maria Elena, Comazzi, Stefano, Giantin, Mery

2013

Overview

Background Canine lymphoma represents the most frequent haematopoietic cancer and it shares some similarities with human non-Hodgkin lymphoma. Matrix metalloproteinases (MMPs) and vascular endothelial growth factor (VEGF) play a coordinated role during invasion and proliferation of malignant cells; however, little is known about their role in canine haematologic malignancies. The aim of this study was to investigate the mRNA and protein expression of VEGF and the most relevant MMPs in canine lymphoma. Lymph node aspirates from 26 B-cell and 21 T-cell lymphomas were collected. The protein expression levels of MMP-9, MMP-2 and VEGF-A were evaluated by immunocytochemistry, and the mRNA levels of MMP-2, MMP-9, MT1-MMP, TIMP-1, TIMP-2, RECK, VEGF-A and VEGF-164 were measured using quantitative RT-PCR. Results MT1-MMP, TIMP-1 and RECK mRNA levels were significantly higher in T-cell lymphomas than in B-cell lymphomas. Higher mRNA and protein levels of MMP-9 and VEGF-A were observed in T-cell lymphomas than in B-cell lymphomas and healthy control lymph nodes. A positive correlation was found between MMP-9 and VEGF-A in T-cell lymphomas. Moreover, MMP-9, MT1-MMP, TIMP-1 and VEGF-A were expressed at the highest levels in high-grade T-cell lymphomas. Conclusions This study provides new information on the expression of different MMPs and VEGF in canine lymphoma, suggesting a possible correlation between different MMPs and VEGF, immunophenotype and prognosis.

• Publisher: BioMed Central,BioMed Central Ltd,Springer Nature B.V
• Subject: Angiogenesis / Animals / B cells / Cell Line, Tumor / cell proliferation / Cloning / Colleges & universities / Diseases / Dog Diseases - metabolism / Dog Diseases - physiopathology / Dogs / gelatinase A / Gene expression / GPI-Linked Proteins - metabolism / GPI-Linked Proteins - physiology / humans / immunocytochemistry / in vivo studies / lymph nodes / Lymph Nodes - metabolism / Lymph Nodes - physiopathology / Lymphoma / Lymphoma - metabolism / Lymphoma - physiopathology / Lymphoma - veterinary / Lymphoma, B-Cell - metabolism / Lymphoma, B-Cell - physiopathology / Lymphoma, B-Cell - veterinary / Lymphoma, T-Cell - metabolism / Lymphoma, T-Cell - physiopathology / Lymphoma, T-Cell - veterinary / Lymphomas / Matrix Metalloproteinase 14 - metabolism / Matrix Metalloproteinase 14 - physiology / Matrix Metalloproteinase 2 - metabolism / Matrix Metalloproteinase 2 - physiology / Matrix Metalloproteinase 9 - metabolism / Matrix Metalloproteinase 9 - physiology / Matrix Metalloproteinases - metabolism / Matrix Metalloproteinases - physiology / Medicine / Medicine & Public Health / messenger RNA / non-Hodgkin lymphoma / Oncology / Pathology / Physiological aspects / prognosis / protein synthesis / Proteins / Real-Time Polymerase Chain Reaction - veterinary / Research Article / Signal transduction / Statistical analysis / Tissue Inhibitor of Metalloproteinase-1 - metabolism / Tissue Inhibitor of Metalloproteinase-1 - physiology / Transgenics / Tumors / Vascular endothelial growth factor / Vascular Endothelial Growth Factor A - metabolism / Vascular Endothelial Growth Factor A - physiology / vascular endothelial growth factors / Veterinary medicine / Veterinary Medicine/Veterinary Science / Zoology