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Analysis of Drug Effects on Primary Human Coronary Artery Endothelial Cells Activated by Serum Amyloid A

by Mrak-Poljšak, Katjuša , Hrušovar, D. , Tomšič, M. , Božič, Borut , Čučnik, Saša , Sodin-Semrl, Snezna , Žigon, Polona , Ogrič, M. , Lakota, Katja

in Acute phase proteins / Adenosine / Ampules / Angiotensin II / Antilipemic agents / Arteriosclerosis / Arthritis / Atherosclerosis / Autoimmune diseases / Captopril / Cardiovascular disease / Cardiovascular diseases / Cell adhesion & migration / Certolizumab pegol / Comparative analysis / Coronary artery / Coronary vessels / Coronary Vessels - cytology / Cytokines / Dexamethasone / Diclofenac / Dihydrofolate reductase / Drug therapy / Drugs / Endothelial cells / Endothelial Cells - cytology / Endothelial Cells - drug effects / Endothelial Cells - metabolism / Endothelium / Enzyme-Linked Immunosorbent Assay / Etanercept / Etoricoxib / Fluvastatin / Glucocorticoids / Humans / Inflammation / Interleukin 6 / Interleukin 8 / Interleukin-6 - metabolism / Interleukin-8 - metabolism / Meloxicam / Methotrexate / Monoclonal antibodies / Mortality / Nonsteroidal anti-inflammatory drugs / Pharmacy / Physiology / Plasminogen Activator Inhibitor 1 - metabolism / Proteins / Rheumatic diseases / Rheumatology / Rosiglitazone / Serum Amyloid A Protein - pharmacology / Thiazolidinediones / Thrombosis / Tumor necrosis factor-α / Vascular Cell Adhesion Molecule-1 - metabolism / Veins & arteries

2018

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Analysis of Drug Effects on Primary Human Coronary Artery Endothelial Cells Activated by Serum Amyloid A

by Mrak-Poljšak, Katjuša , Hrušovar, D. , Tomšič, M. , Božič, Borut , Čučnik, Saša , Sodin-Semrl, Snezna , Žigon, Polona , Ogrič, M. , Lakota, Katja

2018

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Analysis of Drug Effects on Primary Human Coronary Artery Endothelial Cells Activated by Serum Amyloid A

by Mrak-Poljšak, Katjuša , Hrušovar, D. , Tomšič, M. , Božič, Borut , Čučnik, Saša , Sodin-Semrl, Snezna , Žigon, Polona , Ogrič, M. , Lakota, Katja

2018

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Journal Article

Analysis of Drug Effects on Primary Human Coronary Artery Endothelial Cells Activated by Serum Amyloid A

Mrak-Poljšak, Katjuša,

Hrušovar, D.,

Tomšič, M.,

Božič, Borut,

Čučnik, Saša,

Sodin-Semrl, Snezna,

Žigon, Polona,

Ogrič, M.,

Lakota, Katja

2018

Overview

Background. RA patients have a higher incidence of cardiovascular diseases compared to the general population. Serum amyloid A (SAA) is an acute-phase protein, upregulated in sera of RA patients. Aim. To determine the effects of medications on SAA-stimulated human coronary artery endothelial cells (HCAEC). Methods. HCAEC were preincubated for 2 h with medications from sterile ampules (dexamethasone, methotrexate, certolizumab pegol, and etanercept), dissolved in medium (captopril) or DMSO (etoricoxib, rosiglitazone, meloxicam, fluvastatin, and diclofenac). Human recombinant apo-SAA was used to stimulate HCAEC at a final 1000 nM concentration for 24 hours. IL-6, IL-8, sVCAM-1, and PAI-1 were measured by ELISA. The number of viable cells was determined colorimetrically. Results. SAA-stimulated levels of released IL-6, IL-8, and sVCAM-1 from HCAEC were significantly attenuated by methotrexate, fluvastatin, and etoricoxib. Both certolizumab pegol and etanercept significantly decreased PAI-1 by an average of 43%. Rosiglitazone significantly inhibited sVCAM-1 by 58%. Conclusion. We observed marked influence of fluvastatin on lowering cytokine production in SAA-activated HCAEC. Methotrexate showed strong beneficial effects for lowering released Il-6, IL-8, and sVCAM-1. Interesting duality was observed for NSAIDs, with meloxicam exhibiting opposite-trend effects from diclofenac and etoricoxib. This represents unique insight into specific responsiveness of inflammatory-driven HCAEC relevant to atherosclerosis.

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Publisher

Hindawi Publishing Corporation,Hindawi,John Wiley & Sons, Inc,Hindawi Limited,Wiley

Subject

/ Ampules