Asset Details
Do you wish to reserve the book?
Upstream open reading frames dynamically modulate CLOCK protein translation to regulate circadian rhythms and sleep
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Upstream open reading frames dynamically modulate CLOCK protein translation to regulate circadian rhythms and sleep
Do you wish to request the book?
Upstream open reading frames dynamically modulate CLOCK protein translation to regulate circadian rhythms and sleep
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Upstream open reading frames dynamically modulate CLOCK protein translation to regulate circadian rhythms and sleep
2025
Overview
The circadian rhythm is an evolutionarily conserved mechanism with translational regulation increasingly recognized as pivotal in its modulation. In this study, we found that upstream open reading frames (uORFs) are enriched in Drosophila circadian rhythm genes, with particularly conserved uORFs present in core circadian clock genes. We demonstrate evidence that the uORFs of the core clock gene, Clock ( Clk ), rhythmically and substantially attenuate CLK protein translation in Drosophila , with pronounced suppression occurring during daylight hours. Eliminating Clk uORFs leads to increased CLK protein levels during the day and results in a shortened circadian cycle, along with a broad shift in clock gene expression rhythms. Notably, Clk uORF deletion also augments morning sleep by reducing dopaminergic activity. Beyond daily circadian adjustments, Clk uORFs play a role in modulating sleep patterns in response to seasonal daylight variations. Furthermore, the Clk uORFs act as an important regulator to shape the rhythmic expression of a vast array of genes and influence multifaceted physiological outcomes. Collectively, our research sheds light on the intricate ways uORFs dynamically adjust downstream coding sequences to acclimate to environmental shifts.
Publisher
Public Library of Science,Public Library of Science (PLoS)
Subject
/ Circadian Rhythm - physiology
/ Daylight
/ Drosophila melanogaster - genetics
/ Drosophila melanogaster - physiology
/ Drosophila Proteins - genetics
/ Drosophila Proteins - metabolism
/ Genes
/ Insects
/ Open Reading Frames - genetics
/ Protein Biosynthesis - genetics
/ Proteins
/ Research and Analysis Methods
/ RNA
/ Sleep
