Asset Details
Do you wish to reserve the book?
Single-cell transcriptomics captures features of human midbrain development and dopamine neuron diversity in brain organoids
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Single-cell transcriptomics captures features of human midbrain development and dopamine neuron diversity in brain organoids
Do you wish to request the book?
Single-cell transcriptomics captures features of human midbrain development and dopamine neuron diversity in brain organoids
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Single-cell transcriptomics captures features of human midbrain development and dopamine neuron diversity in brain organoids
2021
Overview
Three-dimensional brain organoids have emerged as a valuable model system for studies of human brain development and pathology. Here we establish a midbrain organoid culture system to study the developmental trajectory from pluripotent stem cells to mature dopamine neurons. Using single cell RNA sequencing, we identify the presence of three molecularly distinct subtypes of human dopamine neurons with high similarity to those in developing and adult human midbrain. However, despite significant advancements in the field, the use of brain organoids can be limited by issues of reproducibility and incomplete maturation which was also observed in this study. We therefore designed bioengineered ventral midbrain organoids supported by recombinant spider-silk microfibers functionalized with full-length human laminin. We show that silk organoids reproduce key molecular aspects of dopamine neurogenesis and reduce inter-organoid variability in terms of cell type composition and dopamine neuron formation.
3D brain organoids have been used to investigate human brain development and pathology. Here the authors establish human ventral midbrain organoids coupled with single cell sequencing to study developing and mature dopamine neurons and use silk scaffolding to generate bioengineered brain organoids
Publisher
Nature Publishing Group UK,Nature Publishing Group,Nature Portfolio
Subject
/ 13/100
/ 13/106
/ 13/107
/ 13/31
/ 13/51
/ 14
/ 14/19
/ 45
/ 45/91
/ Brain
/ Brain - growth & development
/ Dopamine
/ Humanities and Social Sciences
/ Humans
/ Laminin
/ Medicinska och farmaceutiska grundvetenskaper
/ Neurons
/ Organoids - growth & development
/ Science
/ Silk
