Asset Details
Do you wish to reserve the book?
Analysis of T-DNA integration and generative segregation in transgenic winter triticale (x TriticosecaleWittmack)
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Analysis of T-DNA integration and generative segregation in transgenic winter triticale (x TriticosecaleWittmack)
Do you wish to request the book?
Analysis of T-DNA integration and generative segregation in transgenic winter triticale (x TriticosecaleWittmack)
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Analysis of T-DNA integration and generative segregation in transgenic winter triticale (x TriticosecaleWittmack)
2012
Overview
Background
While the genetic transformation of the major cereal crops has become relatively routine, to date only a few reports were published on transgenic triticale, and robust data on T-DNA integration and segregation have not been available in this species.
Results
Here, we present a comprehensive analysis of stable transgenic winter triticale cv. Bogo carrying the selectable marker gene
HYGROMYCIN PHOSPHOTRANSFERASE
(
HPT
) and a synthetic
green fluorescent protein
gene (
gfp)
. Progeny of four independent transgenic plants were comprehensively investigated with regard to the number of integrated T-DNA copies, the number of plant genomic integration loci, the integrity and functionality of individual T-DNA copies, as well as the segregation of transgenes in T
1
and T
2
generations, which also enabled us to identify homozygous transgenic lines. The truncation of some integrated T-DNAs at their left end along with the occurrence of independent segregation of multiple T-DNAs unintendedly resulted in a single-copy segregant that is selectable marker-free and homozygous for the
gfp
gene. The heritable expression of
gfp
driven by the maize
UBI-1
promoter was demonstrated by confocal laser scanning microscopy.
Conclusions
The used transformation method is a valuable tool for the genetic engineering of triticale. Here we show that comprehensive molecular analyses are required for the correct interpretation of phenotypic data collected from the transgenic plants.
Publisher
BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC
Subject
/ Agrobacterium tumefaciens - drug effects
/ Agrobacterium tumefaciens - physiology
/ Analysis
/ Biomedical and Life Sciences
/ Cereals
/ Chromosome Segregation - genetics
/ Crops
/ DNA
/ Embryos
/ Gene Expression - drug effects
/ Genes
/ Genetics and crop biotechnology
/ genomics
/ gfp
/ Grain
/ Green Fluorescent Proteins - metabolism
/ Hygromycin B - analogs & derivatives
/ hygromycin phosphotransferase
/ Mutagenesis, Insertional - genetics
/ Plants, Genetically Modified
/ Progeny
/ Seasons
/ Seeds - growth & development
/ T-DNA
/ Winter triticale (x Triticosecale Wittmack)
/ Zea mays
