Journal Article

Clinical iron deficiency disturbs normal human responses to hypoxia

Cheng, Hung-Yuan, Roberts, David J., Nickol, Annabel H., Robbins, Peter A., Ratcliffe, Peter J., Pollard, Karen A., Dorrington, Keith L., Frise, Matthew C., Curtis, M. Kate

2016

Overview

Iron bioavailability has been identified as a factor that influences cellular hypoxia sensing, putatively via an action on the hypoxia-inducible factor (HIF) pathway. We therefore hypothesized that clinical iron deficiency would disturb integrated human responses to hypoxia. We performed a prospective, controlled, observational study of the effects of iron status on hypoxic pulmonary hypertension. Individuals with absolute iron deficiency (ID) and an iron-replete (IR) control group were exposed to two 6-hour periods of isocapnic hypoxia. The second hypoxic exposure was preceded by i.v. infusion of iron. Pulmonary artery systolic pressure (PASP) was serially assessed with Doppler echocardiography. Thirteen ID individuals completed the study and were age- and sex-matched with controls. PASP did not differ by group or study day before each hypoxic exposure. During the first 6-hour hypoxic exposure, the rise in PASP was 6.2 mmHg greater in the ID group (absolute rises 16.1 and 10.7 mmHg, respectively; 95% CI for difference, 2.7-9.7 mmHg, P = 0.001). Intravenous iron attenuated the PASP rise in both groups; however, the effect was greater in ID participants than in controls (absolute reductions 11.1 and 6.8 mmHg, respectively; 95% CI for difference in change, -8.3 to -0.3 mmHg, P = 0.035). Serum erythropoietin responses to hypoxia also differed between groups. Clinical iron deficiency disturbs normal responses to hypoxia, as evidenced by exaggerated hypoxic pulmonary hypertension that is reversed by subsequent iron administration. Disturbed hypoxia sensing and signaling provides a mechanism through which iron deficiency may be detrimental to human health. ClinicalTrials.gov (NCT01847352). M.C. Frise is the recipient of a British Heart Foundation Clinical Research Training Fellowship (FS/14/48/30828). K.L. Dorrington is supported by the Dunhill Medical Trust (R178/1110). D.J. Roberts was supported by R&D funding from National Health Service (NHS) Blood and Transplant and a National Institute for Health Research (NIHR) Programme grant (RP-PG-0310-1004). This research was funded by the NIHR Oxford Biomedical Research Centre Programme.

• Publisher: American Society for Clinical Investigation
• Subject: Adult / Arterial Pressure - physiology / Biomedical research / Cardiac Output / Care and treatment / Case-Control Studies / Cellular signal transduction / Clinical Medicine / Complications and side effects / Development and progression / Echocardiography, Doppler / Enzymes / Erythropoietin - blood / Female / Health aspects / Hepcidins - blood / Homeostasis / Humans / Hypertension, Pulmonary - diagnostic imaging / Hypertension, Pulmonary - etiology / Hypertension, Pulmonary - physiopathology / Hypoxia / Hypoxia - blood / Hypoxia - complications / Hypoxia - physiopathology / Interleukin-6 - blood / Iron - administration & dosage / Iron - blood / Iron Deficiencies / Iron deficiency anemia / Laboratories / Male / Middle Aged / Oxyhemoglobins - metabolism / Prospective Studies / Pulmonary arteries / Pulmonary Artery - diagnostic imaging / Pulmonary Artery - physiopathology / Pulmonary hypertension / Respiration / Rodents / Signal Transduction