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Primary ex vivo cultures of human fallopian tube epithelium as a model for serous ovarian carcinogenesis
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Primary ex vivo cultures of human fallopian tube epithelium as a model for serous ovarian carcinogenesis
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Journal Article
Primary ex vivo cultures of human fallopian tube epithelium as a model for serous ovarian carcinogenesis
2010
Overview
Recent studies suggest that some serous ovarian carcinomas (SOCs) arise from the fallopian tube (FT) epithelium rather than the ovarian surface epithelium. This hypothesis places emphasis on the FT secretory epithelial cell as a cell-of-origin. Herein, we report the development of a novel
ex vivo
primary human FT epithelium culture system that faithfully recapitulates the
in vivo
epithelium, as shown by morphological, ultrastructural and immunophenotypic analyses. Mass spectrometry-based proteomics reveal that these cultures secrete proteins previously identified as biomarkers for ovarian cancer. We also use this culture system to study the response of the FT epithelium to genotoxic stress and find that the secretory cells exhibit a distinct response to DNA damage when compared with neighboring ciliated cells. The secretory cells show a limited ability to resolve the damage over time, potentially leaving them more susceptible to accumulation of additional mutagenic injury. This divergent response is confirmed with
in situ
studies using tissue samples, further supporting the use of this
ex vivo
culture system to investigate FT epithelial pathobiology. We anticipate that this novel culture system will facilitate the study of SOC pathogenesis, and propose that similar culture systems could be developed for other organ site-specific epithelia.
Publisher
Nature Publishing Group UK,Nature Publishing Group
Subject
/ Biological and medical sciences
/ Biomarkers, Tumor - analysis
/ Cell transformation and carcinogenesis. Action of oncogenes and antioncogenes
/ Cell Transformation, Neoplastic - metabolism
/ Cystadenocarcinoma, Serous - metabolism
/ Cystadenocarcinoma, Serous - pathology
/ Female
/ Fundamental and applied biological sciences. Psychology
/ Genetics
/ Gynecology. Andrology. Obstetrics
/ Humans
/ Medicine
/ Molecular and cellular biology
/ Oncology
/ Ovarian Neoplasms - pathology
/ Tumors
