Journal Article

Composition and diversity of bacterial communities in the rhizosphere of the Chinese medicinal herb Dendrobium

Song, Xiaomei, Yuan, Yingdan, Zuo, Jiajia, Zu, Mengting, Liu, Lei

2021

Overview

Background Dendrobium is a precious herbal that belongs to Orchidaceae and is widely used as health care traditional Chinese medicine in Asia. Although orchids are mycorrhizal plants, most research still focuses on endophytes, and there is still large amount unknown about rhizosphere microorganisms. To investigate the rhizosphere microbial community of different Dendrobium species during the maturity stage, we used high-throughput sequencing to analyze microbial community in rhizosphere soil during the maturity stage of three kinds of Dendrobium species. Results In our study, a total of 240,320 sequences and 11,179 OTUs were obtained from these three Dendrobium species. According to the analysis of OTU annotation results, different Dendrobium rhizosphere soil bacteria include 2 kingdoms, 63 phyla, 72 classes, 159 orders, 309 families, 850 genera and 663 species. Among all sequences, the dominant bacterial phyla (relative abundance > 1%) were Proteobacteria, Actinobacteria, Bacteroidetes, Acidobacteria, Firmicutes, Verrucomicrobia, Planctomycetes, Chloroflexi, and Gemmatimonadetes. And through WGCNA analysis, we found the hub flora was also belong to Acidobacteria, Actinobacteria and Proteobacteria. Conclusions We found that the rhizosphere bacterial communities of the three kinds of Dendrobium have significant differences, and that the main species of rhizosphere microorganisms of Dendrobium are concentrated in the Proteobacteria, Actinobacteria, and Bacteroidetes. Moreover, the smaller the bacterial level, the greater the difference among Dendrobium species. These results fill knowledge gaps in the rhizosphere microbial community of Dendrobium and provide a theoretical basis for the subsequent mining of microbial functions and the study of biological fertilizers.

• Publisher: BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC
• Subject: 16 s rRNA / Acidobacteria / Actinobacteria / Agriculture / Algorithms / Annotations / Asia / Bacteria / Bacteria - classification / Bacteria - isolation & purification / Bacteroidetes / Biodiversity / Biological diversity / Biomedical and Life Sciences / Botanical research / Chloroflexi / Composition / Dendrobium / Dendrobium - microbiology / Deoxyribonucleic acid / DNA / Drugs, Chinese Herbal / Endophytes / Environmental aspects / Fertilizers / Firmicutes / flora / Flowers & plants / Gemmatimonadetes / Gene expression / health services / Herbal medicine / High-Throughput Nucleotide Sequencing / Identification and classification / Life Sciences / maturity stage / Medicinal plants / Microbial colonies / microbial communities / Microbial community / Microbiota / Microorganisms / Next-generation sequencing / Orchids / Oriental traditional medicine / Physiological aspects / Planctomycetes / Plant growth / Plant Sciences / Plant-biotic interactions / Plant-soil relationships / Plants, Medicinal - microbiology / Proteobacteria / Relative abundance / Research Article / Rhizosphere / Rhizosphere microorganisms / Rhizosphere soil / Software / soil / Soil bacteria / Soil Microbiology / Soil microorganisms / Soils / Species / Traditional Chinese medicine / Tree Biology / Verrucomicrobia / WGCNA