Journal Article

The effect of royal jelly and tocotrienol-rich fraction along with calorie restriction on hypothalamic endoplasmic reticulum stress and adipose tissue inflammation in diet-induced obese rats

Vafa, Mohammadreza, Mesri Alamdari, Naimeh, Farsi, Farnaz, Shidfar, Farzad, Saidpour, Atoosa, Alivand, Mohammad Reza, Asghari Jafarabadi, Mohammad, Irandoost, Pardis

2020

Overview

Objectives Endoplasmic reticulum (ER) stress causes adipose tissue dysfunction and chronic inflammation in obesity. Royal jelly (RJ) and tocotrienol-rich fraction (TRF) are reported to ameliorate inflammation. However, the improving effects of RJ and TRF on inflammation from ER stress modulating view have not been assessed so far. Hence, we investigated the effect of RJ and TRF on ER stress and some adipose tissue-derived inflammatory markers in the high-fat diet (HFD)-induced obesity. Wistar obese rats randomly allocated into 5 groups: HFD, calorie restriction diet (CRD), RJ + CRD, TRF + CRD, RJ + TRF + CRD. After 8-week intervention, adipose tissues and hypothalamus were dissected and serum was collected. Results RJ reduced glucose-regulated protein-78 (GRP78) expression as ER stress indicator in WAT and hypothalamus compared to CRD. Besides, RJ diminished the expression of inflammatory markers in white adipose tissue (WAT) and also decreased the serum concentration of them. TRF reduced inflammatory markers in the serum without remarkable effects on ER stress. Overall, RJ has protective effect against adipose tissue dysfunction and inflammation then suggested as a therapeutic approach to reduce some obesity-related complications. The impact of TRF in this regard is lower than RJ and limited to systemic inflammation improvement without remarkable changes in adipose tissue inflammation.

• Publisher: BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC
• Subject: Adipocytes / Adipose Tissue / Animals / Biomedical and Life Sciences / Biomedicine / Body fat / Caloric Restriction / Calories / Chronic illnesses / Cytokines / Development and progression / Diet, High-Fat - adverse effects / Endoplasmic reticulum / Endoplasmic Reticulum Stress / Fatty Acids / Gene expression / High fat diet / Hypothalamus / Inflammation / Inflammation - drug therapy / Laboratory animals / Life Sciences / Medicine/Public Health / Nutrient deficiency / Obesity / Obesity - drug therapy / Physiological aspects / Proteins / Rats / Rats, Wistar / Research Note / Rodents / Royal jelly / Tocotrienol-rich fraction / Tocotrienols / Tocotrienols - pharmacology / Tumor necrosis factor-TNF / Variance analysis / Weight control