Journal Article

Inducible clindamycin-resistant and biofilm formation in the Staphylococcus aureus isolated from healthcare worker’s anterior nasal carriage

Pournajaf, Abazar, Mohammadi, Mohsen, Halaji, Mehrdad, Firouzjaei, Mahdi Dadashi, Yaghoubi, Sajad, Salehi, Maryam, Hendizadeh, Peyman

2024

Overview

Objective The purpose of this study is a new update on the resistance profile, Macrolide–Lincosamide–Streptogramin B resistance mechanisms and biofilm formation in the Staphylococcus aureus isolated from health care workers (HCWs) nasal carriage at a children’s teaching hospital in Babol (Northern Iran). Results A total of 143 non-repetitive nasal swab samples were collected from volunteers, where 53.8% (n; 77/143) were HCWs, 33.6% (n; 48/143) medical students, and 12.6% (n; 18/143) resident students. The prevalence of nasal carriers of S. aureus was 22.4% (n; 32/143), among them, 40.6% (n; 13/32) were identified as methicillin-resistant Staphylococcus aureus (MRSA( carriers. Antimicrobial susceptibility testing showed that erythromycin (68.8%, n; 22/32) and ciprofloxacin (15.6%, n; 5/32) had the highest and lowest resistance rate, respectively. The frequency of resistance genes in the strains was as follows; ermC (n; 17/32, 53.1%), ermA (n; 11/32, 34.4%), ermB (n; 6/32, 18.7%), ereA (n; 3/32, 9.4%). Moreover, 50.0% (n; 16/32), 28.1% (n; 9/32) and 21.8% (n; 7/32) of isolates were strongly, weakly and moderately biofilm producer, respectively. Macrolides-lincosamides-streptogramins B (MLSB) antibiotic resistance among S. aureus isolates from HCWs nasal carriage have found significant prevalence rates throughout the globe. It is crucial to remember that the development of biofilms and MLS B antibiotic resistance are both dynamic processes.

• Publisher: BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC
• Subject: Adult / Anti-Bacterial Agents - pharmacology / Antibiotic resistance / Antibiotics / Antimicrobial agents / Biofilm / Biofilms / Biofilms - drug effects / Biofilms - growth & development / Biomedical and Life Sciences / Biomedicine / Carrier State - microbiology / Ciprofloxacin / Clindamycin / Clindamycin - pharmacology / Cross infection / DNA methylation / Drug resistance / Drug resistance in microorganisms / Drug Resistance, Bacterial - genetics / Drug Resistance, Multiple, Bacterial - genetics / Erythromycin / Erythromycin - pharmacology / Female / Gene frequency / Genes / Health aspects / Health care / Health Personnel / Humans / Iran / Life Sciences / Lincosamides / Macrolide–lincosamide–streptogramin B resistance / Male / Medical personnel / Medical research / Medical students / Medicine, Experimental / Medicine/Public Health / Methicillin / Methicillin-Resistant Staphylococcus aureus - drug effects / Methicillin-Resistant Staphylococcus aureus - genetics / Methicillin-Resistant Staphylococcus aureus - isolation & purification / Methicillin-Resistant Staphylococcus aureus - physiology / Microbial mats / Microbial Sensitivity Tests / MRSA / Nasal carriage / Nasal mucosa / Nosocomial infections / Research Note / Risk factors / S. Aureus / Staphylococcal Infections - epidemiology / Staphylococcal Infections - microbiology / Staphylococcus aureus / Staphylococcus aureus - drug effects / Staphylococcus aureus - genetics / Staphylococcus aureus - isolation & purification / Staphylococcus aureus - physiology / Staphylococcus aureus infections / Staphylococcus infections / Statistical analysis / Streptogramin B / Streptogramins / Teaching hospitals