Journal Article

A diverse panel of 755 bread wheat accessions harbors untapped genetic diversity in landraces and reveals novel genetic regions conferring powdery mildew resistance

Swiss National Science Foundation (SNSF)310030B_182833, Keller, Beat, Widrig, Victoria, Paux, Etienne, Universität Zürich [Zürich] = University of Zurich (UZH), Génétique Diversité et Ecophysiologie des Céréales (GDEC) ; Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Clermont Auvergne (UCA), Leber, Rebecca, Heuberger, Matthias, VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS), Sánchez-Martín, Javier, Spanish GovernmentRYC2021-032699-I, Spanish Government PID2022-142651OA-I00, Swiss National Science Foundation (SNSF)310030_204165, Jung, Esther

2024

Overview

Key message A bread wheat panel reveals rich genetic diversity in Turkish, Pakistani and Iranian landraces and novel resistance loci to diverse powdery mildew isolates via subsetting approaches in association studies. Abstract Wheat breeding for disease resistance relies on the availability and use of diverse genetic resources. More than 800,000 wheat accessions are globally conserved in gene banks, but they are mostly uncharacterized for the presence of resistance genes and their potential for agriculture. Based on the selective reduction of previously assembled collections for allele mining for disease resistance, we assembled a trait-customized panel of 755 geographically diverse bread wheat accessions with a focus on landraces, called the LandracePLUS panel. Population structure analysis of this panel based on the TaBW35K SNP array revealed an increased genetic diversity compared to 632 landraces genotyped in an earlier study and 17 high-quality sequenced wheat accessions. The additional genetic diversity found here mostly originated from Turkish, Iranian and Pakistani landraces. We characterized the LandracePLUS panel for resistance to ten diverse isolates of the fungal pathogen powdery mildew. Performing genome-wide association studies and dividing the panel further by a targeted subsetting approach for accessions of distinct geographical origin, we detected several known and already cloned genes, including the Pm2a gene. In addition, we identified 22 putatively novel powdery mildew resistance loci that represent useful sources for resistance breeding and for research on the mildew-wheat pathosystem. Our study shows the value of assembling trait-customized collections and utilizing a diverse range of pathogen races to detect novel loci. It further highlights the importance of integrating landraces of different geographical origins into future diversity studies.

• Publisher: Springer Verlag,CCSD,Springer Berlin Heidelberg,Springer,Springer Nature B.V
• Subject: Agriculture / alleles / Biochemistry / Biological diversity / Biomedical and Life Sciences / Biotechnology / Bread / Care and treatment / Disease resistance / Disease Resistance - genetics / Diseases and pests / fungi / Gene banks / Genes / Genetic aspects / Genetic diversity / Genetic resources / Genetic Variation / Genetics / Genome-wide association studies / Genome-Wide Association Study / Genomes / genotyping / Geographical distribution / Growth / Iran / landraces / Life Sciences / Original / Original Article / Pathogens / Plant Biochemistry / Plant Breeding / Plant Breeding/Biotechnology / Plant Diseases - genetics / Plant Genetics and Genomics / Plants genetics / Population structure / Powdery mildew / Powdery mildew diseases / provenance / single nucleotide polymorphism arrays / Single-nucleotide polymorphism / Triticum - genetics / Vegetal Biology / Wheat
• ISBN: 0011912905000