Asset Details
Do you wish to reserve the book?
Molecular characterization of Glaesserella parasuis strains circulating in North American swine production systems
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Molecular characterization of Glaesserella parasuis strains circulating in North American swine production systems
Do you wish to request the book?
Molecular characterization of Glaesserella parasuis strains circulating in North American swine production systems
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Molecular characterization of Glaesserella parasuis strains circulating in North American swine production systems
2023
Overview
Background
Glaesserella parasuis
is the causative agent of Glässer’s disease in pigs. Serotyping is the most common method used to type
G. parasuis
isolates. However, the high number of non-typables (NT) and low discriminatory power make serotyping problematic. In this study, 218 field clinical isolates and 15
G. parasuis
reference strains were whole-genome sequenced (WGS). Multilocus sequence types (MLST), serotypes, core-genome phylogeny, antimicrobial resistance (AMR) genes, and putative virulence gene information was extracted.
Results
In silico WGS serotyping identified 11 of 15 serotypes. The most frequently detected serotypes were 7, 13, 4, and 2. MLST identified 72 sequence types (STs), of which 66 were novel. The most predominant ST was ST454. Core-genome phylogeny depicted 3 primary lineages (LI, LII, and LIII), with LIIIA sublineage isolates lacking all vtaA genes, based on the structure of the phylogenetic tree and the number of virulence genes. At least one group 1
vtaA
virulence genes were observed in most isolates (97.2%), except for serotype 8 (ST299 and ST406), 15 (ST408 and ST552) and NT (ST448). A few group 1
vtaA
genes were significantly associated with certain serotypes or STs. The putative virulence gene
lsgB
, was detected in 8.3% of the isolates which were predominantly of serotype 5/12. While most isolates carried the
bcr
,
ksgA
, and
bacA
genes, the following antimicrobial resistant genes were detected in lower frequency;
blaZ
(6.9%),
tetM
(3.7%),
spc
(3.7%),
tetB
(2.8%),
bla-ROB-1
(1.8%),
ermA
(1.8%),
strA
(1.4%),
qnrB
(0.5%), and
aph3''Ia
(0.5%).
Conclusion
This study showed the use of WGS to type
G. parasuis
isolates and can be considered an alternative to the more labor-intensive and traditional serotyping and standard MLST. Core-genome phylogeny provided the best strain discrimination. These findings will lead to a better understanding of the molecular epidemiology and virulence in
G. parasuis
that can be applied to the future development of diagnostic tools, autogenous vaccines, evaluation of antibiotic use, prevention, and disease control.
Publisher
BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC
Subject
